Human Anti-ECD Recombinant Antibody (clone mAb1) (CAT#: PABL-459)
Recombinant Human Antibody (mAb1) is capable of binding to ECD, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-ECD mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-ECD proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody inhibits glucagon receptor by occluding a surface extending across the entire hormone-binding cleft.
Specific Inquiry
Figure 1 Western blot analysis of stable 293 cells expressing WT or G40S GCGR, probed with anti-GCGR mAb1.
WT or G40S -ve refers to cells incubated without anti-GCGR.
Mukund, S., Shang, Y., Clarke, H. J., Madjidi, A., Corn, J. E., Kates, L., ... & Zhang, Y. (2013). Inhibitory mechanism of an allosteric antibody targeting the glucagon receptor. Journal of Biological Chemistry, 288(50), 36168-36178.
Figure 2 FACS analysis of stable 293 cells expressing WT or G40S GCGR using anti-GCGR mAb1.
WT or G40S -ve refers to cells incubated without anti-GCGR.
Mukund, S., Shang, Y., Clarke, H. J., Madjidi, A., Corn, J. E., Kates, L., ... & Zhang, Y. (2013). Inhibitory mechanism of an allosteric antibody targeting the glucagon receptor. Journal of Biological Chemistry, 288(50), 36168-36178.
Specifications
- Immunogen
- Human ecdysoneless homolog (Drosophila)
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human ECD
- Species Reactivity
- Human
- Clone
- mAb1
- Applications
- ELISA, WB, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- ECD; ecdysoneless homolog (Drosophila); GCR2; HSGT1; protein SGT1; suppressor of GCR2; protein ecdysoneless homolog; suppressor of S. cerevisiae gcr2;
- Gene ID
- 11319
- UniProt ID
- O95905
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-460 | Human Anti-ECD Recombinant Antibody (clone mAb23) | ELISA, WB, FuncS | Human IgG |
| MOB-1843MZ | Recombinant Mouse Anti-Human ECD Antibody | ELISA, WB | Mouse antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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