Human Anti-ECD Recombinant Antibody (clone mAb1) (CAT#: PABL-459)
Recombinant Human Antibody (mAb1) is capable of binding to ECD, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-ECD mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-ECD proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody inhibits glucagon receptor by occluding a surface extending across the entire hormone-binding cleft.
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Figure 1 Western blot analysis of stable 293 cells expressing WT or G40S GCGR, probed with anti-GCGR mAb1.
WT or G40S -ve refers to cells incubated without anti-GCGR.
Mukund, S., Shang, Y., Clarke, H. J., Madjidi, A., Corn, J. E., Kates, L., ... & Zhang, Y. (2013). Inhibitory mechanism of an allosteric antibody targeting the glucagon receptor. Journal of Biological Chemistry, 288(50), 36168-36178.
Figure 2 FACS analysis of stable 293 cells expressing WT or G40S GCGR using anti-GCGR mAb1.
WT or G40S -ve refers to cells incubated without anti-GCGR.
Mukund, S., Shang, Y., Clarke, H. J., Madjidi, A., Corn, J. E., Kates, L., ... & Zhang, Y. (2013). Inhibitory mechanism of an allosteric antibody targeting the glucagon receptor. Journal of Biological Chemistry, 288(50), 36168-36178.
Specifications
- Immunogen
- Human ecdysoneless homolog (Drosophila)
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human ECD
- Species Reactivity
- Human
- Clone
- mAb1
- Applications
- ELISA, WB, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- ECD; ecdysoneless homolog (Drosophila); GCR2; HSGT1; protein SGT1; suppressor of GCR2; protein ecdysoneless homolog; suppressor of S. cerevisiae gcr2;
- Gene ID
- 11319
- UniProt ID
- O95905
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-460 | Human Anti-ECD Recombinant Antibody (clone mAb23) | ELISA, WB, FuncS | Human IgG |
| MOB-1843MZ | Recombinant Mouse Anti-Human ECD Antibody | ELISA, WB | Mouse antibody |
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