Is the Trastuzumab antibody suitable for use in flow cytometry analysis?

Yes, the Trastuzumab antibody (TP-083CL) is suitable for use in flow cytometry analysis. It is designed to specifically bind to its target, making it suitable for detecting target-expressing cells in flow cytometry.

What are the recommended storage conditions for the Trastuzumab antibody?

The recommended storage condition for the Trastuzumab antibody (TP-083CL) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

Can the Trastuzumab antibody be used in Western blotting?

Yes, the Trastuzumab antibody (TP-083CL) can be used in Western blotting. It provides specific binding to its target, allowing for reliable detection in Western blot assays.

Is the Trastuzumab antibody effective in ELISA applications?

Yes, the Trastuzumab antibody (TP-083CL) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of its target.

What is the optimal dilution for using the Trastuzumab antibody in immunofluorescence?

The optimal dilution for using the Trastuzumab antibody (TP-083CL) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

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IGHG1
Human Anti-ERBB2 Rcombinant Antibody (TP-083CL)

Human Anti-ERBB2 Rcombinant Antibody (TP-083CL) (CAT#: TP-083CL)

A recombinant IgG1 kappa, humanized monoclonal antibody that selectively binds with high affinity in a cell-based assay (Kd = 5 nM) to the extracellular domain of the human epidermal growth factor receptor protein. Produced in CHO cell culture.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
Publications
COA

SPR

Figure 1 Trastuzumab (TP-083CL) in SPR

Figure 1 Trastuzumab (TP-083CL) in SPR

SPR assays of TP-083CL.
The affinity of Trastuzumab (TP-083CL) was determined by SPR.
Captured TP-083CL on CM5 Chip via Anti-Human IgG (Fc) can bind Human ERBB2 protein with an affinity constant of 1.45 nM as determined in a SPR assay (Biacore 8K).

Normal Tissue

Figure 1 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_9_3.jpg

Figure 2 Liver
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_9_4.jpg

Figure 3 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_8_5.jpg

Figure 4 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_5_6.jpg

RNA Expression

Figure 5 RNA cell line category: Group enriched (Karpas-707, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000211896-IGHG1

Datasheet

MSDS

Peer-reviewed Publications

Our products have been used extensively by scientists all over the world to accelerate their life science research. Here, we present a collection of publications that cite this product.

Chen, Wu, et al. "Performance of Optimized Wide Pore Superficially Porous Particles for Separation of Proteins and Immunoglobulin G Antibodies." Journal of chromatographic science 56.5 (2018): 416-424.

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Type
Biotech

Target

Function
Humanized mAb that binds Her2/Neu cell surface receptor and controls cancer cell growth

Related Resources

Biosimilar Overview
Citations

Please refer to Trastuzumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Trastuzumab.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "IGHG1"

Select a product category from the dropdown menu below to view related products.

Please select product type

IgG Antibody Products Rabbit Monoclonal Antibody Products Epitope-Specific Antibody Products Extracellular Vesicle (EV) Antibody Products

CAT	Product Name	Application	Type
MOB-1117z	Mouse Anti-IGHG1 Recombinant Antibody (clone 5C2)	FC, IF, IHC, WB	Mouse IgG1, κ
MOB-034LC	Mouse Anti-IGHG1 Recombinant Antibody (clone YC121)	ELISA, WB, IHC	Mouse IgG
VS3-FY2150	Rabbit Anti-IGHG1 Recombinant Antibody (clone R29-3R-6)	WB	Rabbit IgG

CAT	Product Name	Application	Type
MOR-1394	Rabbit Anti-IGHG1 Recombinant Antibody (clone DS1394AB)	ICC, IHC, FC, ELISA	Rabbit IgG
MOR-1771	Rabbit Anti-IGHG1 Recombinant Antibody (clone DS1771AB), PE	FC	Rabbit IgG

CAT	Product Name	Application	Type
EPAF-0577CQ	Mouse Anti-IGHG1 Recombinant Antibody (clone B3-18)	ELISA	Mouse IgM

CAT	Product Name	Application	Type
VS-0425-YC67	Recombinant Anti-IGHG1 Vesicular Antibody, EV Displayed (VS-0425-YC67)	ELISA, FC, Cell-uptake

Customer Reviews and Q&As

Customer Review Q&As

Laura Brown
Ideal for HER2 Research

The Trastuzumab has been excellent for our HER2 research. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.

03.Dec,23

Emily Lewis
Reliable in Immunoassays

We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for HER2 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.

09.Aug,22

John Carter
Consistent and Effective

This antibody has proven to be consistent and effective in our various assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for HER2 have been particularly beneficial.

26.Sep,21

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TP-083CL, RRID: AB_3112050)

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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