Hi-Affi™ Rabbit Anti-ESR1 Recombinant Antibody (clone DS1201AB) (CAT#: MOR-1201)
Recombinant Rabbit anti-ESR1 monoclonal antibody for IHC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1134_D1_1_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1134_D1_1_selected.jpg
Figure 2 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1423_D4_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1423_D4_1_blue_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1_G2_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1_G2_1_blue_red_green.jpg
Figure 4 Endometrium
(Cells in endometrial stroma
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37/288_B_3_3.jpg
(Cells in endometrial stroma
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37/288_B_3_3.jpg
Figure 5 RNA cell line category: Group enriched (MCF7, T-47d)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000091831-ESR1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000091831-ESR1
Specifications
- Immunogen
- Recombinant protein of ER α amino acids 1-300
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Clone
- DS1201AB
- Applications
- IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Estrogen Receptor 1; Nuclear Receptor Subfamily 3 Group A Member 1; Estradiol Receptor; ER-Alpha; NR3A1; ESR; ER; Estrogen Receptor Alpha Splice Variant, ERalphai45bL; Estrogen Receptor Alpha Splice Variant, ERalphai45bS; Estrogen Receptor Alpha Splice Variant, ERalphaDup5; Estrogen Receptor Alpha Splice Variant, ERalphai45a; Estrogen Receptor Alpha Splice Variant, ERalphai45c; Estrogen Receptor Alpha Splice Variant, ERalphai56; Estrogen Receptor Alpha Splice Variant, ERalphai67; Estrogen Receptor Alpha Splice Variant, CTERP-1; Estrogen Receptor Alpha E1-N2-E2-1-2; Estrogen Receptor Alpha E1-E2-1-2; Estrogen Nuclear Receptor Alpha; ESTRR; ESRA; ERA
- Gene ID
- 2099
- UniProt ID
- P03372
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-898-VHH | Recombinant Anti-Human ESR1 VHH Single Domain Antibody | FA, FC, ICC, ELISA | Llama VHH |
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