Human Anti-F9 Recombinant Antibody (clone 10C12); Fab Fragment (CAT#: PFBC-044)

Recombinant Human Antibody (10C12) Fab Fragment is specific to Factor IX, expressed in Chinese Hamster Ovary cells (CHO).


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Figure 1 F.IX activation by F.XIa and by the tissue factor:factor VIIa (TF:F.VIIa) complex.

Figure 1 F.IX activation by F.XIa and by the tissue factor:factor VIIa (TF:F.VIIa) complex.

F.IX (400 nM) was incubated with 10C12 (filled symbols) or a control antibody (NTN: anti-neurturin) (open symbols) in HBSA-5 mM CaCl₂. (A) 1 nM F.XIa was added to start the reaction. (B) Relipidated TF:F.VIIa (4 nM:1 nM) (circles) or membraneTF:F.VIIa (150 μg/ml:1nM) (squares) was added to start the reaction. At defined time intervals reaction aliquots were quenched in EDTAethyleneglycol and F.IXa amidolytic activity in each sample was determined after adding F.IXa substrate #299. The inhibition of the rates of F.IXa generation were expressed as fractional rates (vᵢ/vo) ± SD of 4 independent experiments (except for the NTN control in Fig. 1A where n = 3)

Refino, C. J., Himber, J., Burcklen, L., Moran, P., Peek, M., Suggett, S., ... & Kirchhofer, D. (1999). A human antibody that binds to the γ-carboxyglutamic acid domain of factor IX is a potent antithrombotic in vivo. Thrombosis and haemostasis, 82(09), 1188-1195.

Figure 2 Measurement of activated partial thromboplastin time (APTT) and prothrombin time (PT) in plasma of guinea pig and rat.

Figure 2 Measurement of activated partial thromboplastin time (APTT) and prothrombin time (PT) in plasma of guinea pig and rat.

10C12 was diluted in citrated plasma of guinea pig and rat. After an incubation of 10 min, human relipidated tissue factor (Innovin) or Actin FS were added to start the PT (open symbols) and APTT (filled symbols) reaction, respectively. The effects on clotting were expressed as fold prolongation of control plasma clotting times. Diamonds = guinea pig; circles = rat.

Refino, C. J., Himber, J., Burcklen, L., Moran, P., Peek, M., Suggett, S., ... & Kirchhofer, D. (1999). A human antibody that binds to the γ-carboxyglutamic acid domain of factor IX is a potent antithrombotic in vivo. Thrombosis and haemostasis, 82(09), 1188-1195.

Figure 3 Effects of 10C12 on cyclic flow variations (CFVs) in guinea pig arterial thrombosis model.

Figure 3 Effects of 10C12 on cyclic flow variations (CFVs) in guinea pig arterial thrombosis model.

10C12 and controls were given by intravenous bolus administration 15 min prior to the initiation of CFVs in the carotid artery. The number of CFVs during a 40 min period was recorded and the thrombosis index calculated as the ratio of CFVs divided by the number of applied pinches. ** p<0.01, *** p <0.001 versus control by Mann-Whitney U-test following determination of significant differences between multiple groups in KruskalWallis test

Refino, C. J., Himber, J., Burcklen, L., Moran, P., Peek, M., Suggett, S., ... & Kirchhofer, D. (1999). A human antibody that binds to the γ-carboxyglutamic acid domain of factor IX is a potent antithrombotic in vivo. Thrombosis and haemostasis, 82(09), 1188-1195.

Figure 4 Blood loss from a standardized puncture wound of rabbit tibias for three consecutive 30-minute periods.

Figure 4 Blood loss from a standardized puncture wound of rabbit tibias for three consecutive 30-minute periods.

The treatment groups were saline (open bar), 10C12 administered as a single bolus of 30 μg/kg (hatched bar), and heparin administered as a bolus of 100 U/kg followed by a continuous infusion of 1 U · kg⁻¹ · min⁻¹ for 30 minutes (closed bar). N5 per treatment. Times were 0 to 30 minutes (before treatment), 30 to 60 minutes (post-treatment but during the heparin infusion), 60 to 90 minutes (post-treatment but after termination of heparin infusion). *P≤0.05 versus saline control.

Refino, C. J., Jeet, S., DeGuzman, L., Bunting, S., & Kirchhofer, D. (2002). A human antibody that inhibits factor IX/IXa function potently inhibits arterial thrombosis without increasing bleeding. Arteriosclerosis, thrombosis, and vascular biology, 22(3), 517-522.


Specifications

  • Immunogen
  • Human coagulation factor IX
  • Host Species
  • Human
  • Type
  • Human Fab
  • Specificity
  • Human F9
  • Species Reactivity
  • Human
  • Clone
  • 10C12
  • Applications
  • Block

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody Fab fragment 10C12 has been reported in applications of Blocking, Inhibition. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.

Target

  • Alternative Names
  • F9; coagulation factor IX; FIX; P19; PTC; HEMB; THPH8; F9 p22; FIX F9; factor 9; factor IX F9; Christmas factor; plasma thromboplastic component; plasma thromboplastin component

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone 10C12"

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Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1230 Hi-Affi™ Rabbit Anti-F9 Recombinant Antibody (clone DS1230AB) WB, ICC, FC Rabbit IgG

Epitope-Specific Antibody

CAT Product Name Application Type
EPAF-0574CQ Recombinant Human Anti-Human F9 Antibody (10C12) ELISA IgG1

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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