This product is a recombinant Human antibody that recognizes HIV-1 Env. The antibody was purified by affinity chromatography. It can be used for Neut and other applications.
Figure 1 Human Anti-HIV-1 Env Recombinant Antibody (PABL-519) in WB
Western blot analysis was performed with 1 µg (lane 1), 2 µg(lane 2) recombinant HIV-1 Env onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with PABL-519 at a dilution of 1:250 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:2,000 for one hour. Chemiluminescent was detected.
Figure 2 Human Anti-HIV-1 Env Recombinant Antibody (PABL-519) in Dot Blot
Dot Blot analysis of PABL-519 was performed by coating with HIV-1 Env antigen.
PABL-519 incubation concentration: 4 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Figure 3 Human Anti-HIV-1 Env Recombinant Antibody (PABL-519) in ELISA
ELISA analysis of PABL-519 was performed by coating with recombinant HIV-1 Env antigen. Then blocked with BSA, and incubated with PABL-519 at a starting concentration of 2.5 µg/mL. The HRP-conjugated goat anti-human IgG was used as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
NABG-057 | Recombinant Anti-HIV-1 env VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-474 | Recombinant Human Anti-HIV-1 Env Antibody | WB, FuncS | IgG |
PABL-379 | Human Anti-HIV-1 Env Recombinant Antibody (clone VRC0) | Neut, FuncS | Human IgG |
MRO-781CQ | Mouse Anti-HIV-1 Env Recombinant Antibody (clone 3H9/D6) | ELISA, WB | Mouse IgG |
MRO-2796CQ | Human Anti-HIV-1 Env Recombinant Antibody (clone 3D5) | Neut | Human IgG |
MRO-2797CQ | Human Anti-HIV-1 Env Recombinant Antibody (clone 1B1) | Neut | Human IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-137 | Human Anti-HIV-1 Env Recombinant Antibody (clone PGT124); Fab Fragment | WB, Neut, FuncS | Human Fab |
PFBL-141 | Human Anti-HIV-1 Env Recombinant Antibody (clone 35O22); Fab Fragment | WB, ELISA, Neut, FuncS | Human Fab |
PFBL-143 | Human Anti-HIV-1 Env Recombinant Antibody (clone 9H+3L); Fab Fragment | WB, ELISA, IF, FuncS | Human Fab |
PFBL-144 | Human Anti-HIV-1 Env Recombinant Antibody (clone C11); Fab Fragment | WB, ELISA, FuncS | Human Fab |
PFBL-145 | Human Anti-HIV-1 Env Recombinant Antibody; Fab Fragment (PFBL-145) | ELISA, Neut, FuncS | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBC-420 | Human Anti-HIV-1 Env Recombinant Antibody (clone CAP228-3D); scFv Fragment | Neut | Human scFv |
PABJ-0103-S(P) | Rabbit Anti-HIV-1 Env Recombinant Antibody (clone E70); scFv Fragment | ELISA, Neut | Rabbit scFv |
PABJ-0140-S(P) | Human Anti-HIV-1 Env Recombinant Antibody; scFv Fragment (PABJ-0140-S(P)) | ELISA, Neut | Human scFv |
PABJ-0141-S(P) | Human Anti-HIV-1 Env Recombinant Antibody; scFv Fragment (PABJ-0141-S(P)) | ELISA, Neut | Human scFv |
PABJ-0147-S(P) | Human Anti-HIV-1 Env Recombinant Antibody (clone ACS202); scFv Fragment | ELISA, Neut | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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