Human Anti-HIV-1 Env Recombinant Antibody (PABL-519) (CAT#: PABL-519)
This product is a recombinant Human antibody that recognizes HIV-1 Env. The antibody was purified by affinity chromatography. It can be used for Neut and other applications.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Human Anti-HIV-1 Env Recombinant Antibody (PABL-519) in WB
Western blot analysis was performed with 1 µg (lane 1), 2 µg(lane 2) recombinant HIV-1 Env onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with PABL-519 at a dilution of 1:250 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:2,000 for one hour. Chemiluminescent was detected.
DB
Figure 2 Human Anti-HIV-1 Env Recombinant Antibody (PABL-519) in Dot Blot
Dot Blot analysis of PABL-519 was performed by coating with HIV-1 Env antigen.
PABL-519 incubation concentration: 4 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
ELISA
Figure 3 Human Anti-HIV-1 Env Recombinant Antibody (PABL-519) in ELISA
ELISA analysis of PABL-519 was performed by coating with recombinant HIV-1 Env antigen. Then blocked with BSA, and incubated with PABL-519 at a starting concentration of 2.5 µg/mL. The HRP-conjugated goat anti-human IgG was used as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- HIV-1 Env
- Species Reactivity
- HIV-1
- Applications
- Neut
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160, precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain
- Gene ID
- 155971
- UniProt ID
- P04578
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-057 | Recombinant Anti-HIV-1 env VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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