Anti-HIV-1 gp120 Recombinant Antibody (G3-136) (CAT#: MRO-152LC)
This product is a recombinant mouse anti-HIV-1 monoclonal antibody. G3-136 specifically binds to gp120 and can be potentially used in the treatment of acquired immunodeficiency syndrome (AIDS), a condition in humans characterized by clinical features including wasting syndromes, central nervous system degeneration and profound immunosuppression that results in life-threatening opportunistic infections and malignancies.
Specific Inquiry
Neut
Figure 1. Neutralization of HIV-1 by G3-136.
Inhibition of infection of CEM-SS cells by laboratory HIV-1 isolates IIIB (●), MN (○), and RF (△).
Fung, M. S., Sun, C. R., Gordon, W. L., Liou, R. S., Chang, T. W., Sun, W. N., ... & Ho, D. D. (1992). Identification and characterization of a neutralization site within the second variable region of human immunodeficiency virus type 1 gp120. Journal of virology, 66(2), 848-856.
ELISA
Figure 2. Inhibitory activity of sCD4 on the binding of biotinylated MAbs to captured HIV-1 IIIB gpl20 in ELISA.
These biotinylated antibodies, including G3-136 (△), was used at dilutions which gave halfmaximal binding to the captured gpl20.
Fung, M. S., Sun, C. R., Gordon, W. L., Liou, R. S., Chang, T. W., Sun, W. N., ... & Ho, D. D. (1992). Identification and characterization of a neutralization site within the second variable region of human immunodeficiency virus type 1 gp120. Journal of virology, 66(2), 848-856.
Specifications
- Host Species
- Mouse
- Type
- Mouse antibody
- Specificity
- HIV-1
- Clone
- G3-136
- Applications
- Neut, ELISA
- Related Disease
- AIDS
Applications
- Application Notes
- The G3-136 antibody has been reported in applications of Neut, ELISA.
Neut: 50 ul of diluted MAb was mixed with 50 ,ul of viral culture supernatant containing 200 syncytium-forming units (SFU) of HIV-1 IIIB, MN, or RF and incubated for 1h at room temperature. The mixtures were added into microculture wells containing 5 x 104 DEAE-dextrantreated CEM-SS cells, and the cell cultures were maintained in 5% CO2 at 37°C for 3 to 4 days. The syncytia were then enumerated under an inverted microscope. The neutralizing activity was expressed as ID50, defined as the concentration required to achieve 50% inhibition of the infection (i.e., Vn/Vo = 50%), where Vn is the number of SFU in the test wells and Vo is the number of SFU in the control without test antibodies.
ELISA: Wells of Immulon 2 microdilution plates were coated overnight at room temperature with 100 ul of purified gpl20 (0.1 p.g/ml) in phosphate-buffered saline (PBS). The wells were then treated with 5% BLOTTO in PBS for 1 h at room temperature and washed with PBS containing 0.05% Tween 20 (PBST). Next, 100 ,ul of test culture supernatant was added to each well for 1 h at room temperature.
It's recommended that the optimal antibody concentration, dilution, incubation time etc. are best to be carefully titrated in specific assays. For details, refer to Published Data.
Target
- Alternative Names
- Human immunodeficiency virus type 1; HIV-1; Envelope glycoprotein GP120; gp120; HIV envelope
- Gene ID
- 155971
- UniProt ID
- P04578
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-135 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PGT135) | ELISA, Neut | Human IgG |
| PABL-142 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 412d) | Neut, WB, ELISA, FuncS | Human IgG |
| PABL-155 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC03) | WB, ELISA, Neut, FuncS | Human IgG |
| PABL-156 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC-CH31) | WB, ELISA, Neut, FuncS | Human IgG |
| PABL-160 | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 59.1) | WB, ELISA, FuncS | Mouse IgG1, κ |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-135 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PGT135); Fab Fragment | ELISA, Neut | Human Fab |
| PFBL-142 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 412d); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| PFBL-155 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC03); Fab Fragment | WB, ELISA, Neut, FuncS | Human Fab |
| FAMAB-0177CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone BAT123); Fab Fragment | Neut | Chimeric (mouse/human) Fab |
| FAMAB-0178CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone C beta 1); Fab Fragment | WB, Neut | Chimeric (mouse/human) Fab |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-028LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91C2) | ELISA | Single domain antibody |
| MRO-029LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91F10) | ELISA | Single domain antibody |
| MRO-030LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91E1) | ELISA | Single domain antibody |
| MRO-031LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91B5) | ELISA | Single domain antibody |
| MRO-032LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91H9) | ELISA | Single domain antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-041LC | Anti-HIV-1 gp120 Recombinant Antibody (D59/A2) | ELISA | Mouse antibody |
| MRO-042LC | Anti-HIV-1 gp120 Recombinant Antibody (F58/H3) | WB, ELISA | Mouse antibody |
| MRO-043LC | Anti-HIV-1 gp120 Recombinant Antibody (P1/D12) | ELISA | Mouse antibody |
| MRO-044LC | Anti-HIV-1 gp120 Recombinant Antibody (P4/D10) | ELISA | Mouse antibody |
| MRO-045LC | Anti-HIV-1 gp120 Recombinant Antibody (A47/B1) | ELISA | Mouse antibody |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-125LC | Anti-HIV-1 gp120 Recombinant Antibody (N6) | Neut, ELISA | Human antibody |
| MRO-130LC | Human Anti-HIV-1 gp120 Recombinant Antibody (MRO-130LC) | ELISA, Neut, SPR | Human IgG |
| MRO-136LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900973) | ELISA | Human antibody |
| MRO-137LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900972) | ELISA | Human antibody |
| MRO-138LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900990) | ELISA | Human antibody |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-135LC | Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) | ELISA, WB, Neut | Chimeric antibody (mouse/human) |
| MRO-135LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
| MRO-135LC-F(E) | Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4220 | Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) | WB, ELISA | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2788CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 570-D); scFv Fragment | ELISA | Human scFv |
| MRO-2792CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone gVRC-H10dC38/VRC01L); scFv Fragment | ELISA, Neut | Human scFv |
| MRO-2793CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone gVRC-H1dC38/VRC01L); scFv Fragment | ELISA, Neut | Human scFv |
| MRO-2794CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone gVRC-H2dC38/VRC01L); scFv Fragment | ELISA, Neut | Human scFv |
| MRO-2795CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone gVRC-H10dC38/gVRC-L1dC38); scFv Fragment | Neut | Human scFv |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-101-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment | WB, ELISA, FuncS | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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