Anti-HIV-1 gp120 Recombinant Antibody (G3-136) (CAT#: MRO-152LC)

• Host Species
• Mouse

• Type
• Mouse antibody

• Specificity
• HIV-1

• Clone
• G3-136

• Applications
• Neut, ELISA

• Related Disease
• AIDS

• Application Notes
• The G3-136 antibody has been reported in applications of Neut, ELISA.
  Neut: 50 ul of diluted MAb was mixed with 50 ,ul of viral culture supernatant containing 200 syncytium-forming units (SFU) of HIV-1 IIIB, MN, or RF and incubated for 1h at room temperature. The mixtures were added into microculture wells containing 5 x 104 DEAE-dextrantreated CEM-SS cells, and the cell cultures were maintained in 5% CO2 at 37°C for 3 to 4 days. The syncytia were then enumerated under an inverted microscope. The neutralizing activity was expressed as ID50, defined as the concentration required to achieve 50% inhibition of the infection (i.e., Vn/Vo = 50%), where Vn is the number of SFU in the test wells and Vo is the number of SFU in the control without test antibodies.
  ELISA: Wells of Immulon 2 microdilution plates were coated overnight at room temperature with 100 ul of purified gpl20 (0.1 p.g/ml) in phosphate-buffered saline (PBS). The wells were then treated with 5% BLOTTO in PBS for 1 h at room temperature and washed with PBS containing 0.05% Tween 20 (PBST). Next, 100 ,ul of test culture supernatant was added to each well for 1 h at room temperature.
  It's recommended that the optimal antibody concentration, dilution, incubation time etc. are best to be carefully titrated in specific assays. For details, refer to Published Data.

• Alternative Names
• Human immunodeficiency virus type 1; HIV-1; Envelope glycoprotein GP120; gp120; HIV envelope

• Gene ID
• 155971

• UniProt ID
• P04578

• MRO-152LC-S(P)
• Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (G3-136)

• MRO-152LC-F(E)
• Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (G3-136)

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