Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 0.5β) (CAT#: PABZ-061)
Recombinant Mouse Antibody (0. 5β) is capable of binding to HIV-1 gp120, expressed in mammalian cells. The affinity of 0. 5β for the V3 peptide P1053 (RKSIRIQRGPGRAFVTIG) was found to be comparable to its affinity for the intact glycoprotein.
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Figure 1 Titration of the 0.5β Fv fragment with wild-type peptide monitored by fluorescence enhancement.
The concentration of the 0.5β Fv fragment was 0.5 μM.
Faiman, G. A., Levy, R., Anglister, J., & Horovitz, A. (1996). Contribution of Arginine Residues in the RP135 Peptide Derived from the V3 Loop of gp120 to Its Interaction with the Fv Fragment of the 0.5 β HIV-1 Neutralizing Antibody. Journal of Biological Chemistry, 271(23), 13829-13833.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Tested positive against HIV-1 gp120
- Species Reactivity
- HIV-1
- Clone
- 0.5β
- Applications
- Neut Assay-Dependent
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody 0.5β has been reported in application of Neut. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
- Alternative Names
- ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160, precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain
- Gene ID
- 155971
- UniProt ID
- P04578
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Humanized Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-135 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PGT135) | ELISA, Neut | Human IgG |
| PABL-142 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 412d) | Neut, WB, ELISA, FuncS | Human IgG |
| PABL-155 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC03) | WB, ELISA, Neut, FuncS | Human IgG |
| PABL-156 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC-CH31) | WB, ELISA, Neut, FuncS | Human IgG |
| PABL-160 | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 59.1) | WB, ELISA, FuncS | Mouse IgG1, κ |
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