Human Anti-HIV-1 gp120 Recombinant Antibody (clone F105) (CAT#: PABW-160)
Recombinant Human Antibody (F105) is capable of binding to HIV-1 gp120, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti- HIV-1 gp120 mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti- HIV-1 gp120 proteins mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. F105 is a neutralizing, CD4–binding site (CD4BS) antibody, binding to gp120 occurred primarily through heavy-chain interactions, which were similar in many respects to those of CD4. F105 recognized conformational shifts in the position of the V1/V2 stem, resulting in a V1/V2 orientation poorly compatible with the functional viral spike.
Specific Inquiry
WB
Figure 1 Efficient cleavage of 4-2.J41 Env on the cell surface.
(C) Western blot analysis of immunoprecipitated 4-2.J41 Env protein from plasmamembrane fraction. Proteins from the plasma membrane fraction of Env transfected 293T cells were immunoprecipitated with VRC01, PGT151 and F105 antibodies and analyzed by Western blot using HIVIG as probe. M = molecular weight marker.
Boliar, S., Das, S., Bansal, M., Shukla, B. N., Patil, S., Shrivastava, T., ... & Chakrabarti, B. K. (2015). An efficiently cleaved HIV-1 clade C Env selectively binds to neutralizing antibodies. PloS one, 10(3), e0122443.
Specifications
- Immunogen
- HIV-1 gp120 protein
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Tested positive against HIV-1 gp120 protein
- Species Reactivity
- HIV-1
- Clone
- F105
- Applications
- FC, Neut, WB, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The ENV antibody has been reported in applications of Fluorescence activated Cell Sorting, Neutralization, Western Blot, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160, precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain
- Gene ID
- 155971
- UniProt ID
- P04578
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Humanized Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-135 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PGT135); scFv Fragment | ELISA, Neut | Human scFv |
| PSBL-142 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 412d); scFv Fragment | WB, ELISA, FuncS | Human scFv |
| PSBL-155 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC03); scFv Fragment | WB, ELISA, Neut, FuncS | Human scFv |
| PSBL-156 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC-CH31); scFv Fragment | WB, ELISA, Neut, FuncS | Human scFv |
| PSBL-160 | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 59.1); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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