Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC01) (CAT#: PABL-171)
Recombinant Human Antibody (VRC01) is capable of binding to HIV-1 gp120, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV-1 gp120 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HIV-1 gp120 mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Specific Inquiry
Figure 1 Binding specificity of VRC06 to gp120 defined by ELISA.
(A) VRC06 binding to YU2 gp120 was sensitive to gp120 mutations in both the CD4bs (D368R) and the CoRbs (I420R). Control MAbs included CD4bs ligands (VRC01 and b12) and CoRbs MAb 17b. (B) VRC06 competed with biotin-labeled CD4bs and CoRbs MAb binding to YU2 gp120. (C) sCD4 competed with CD4bs MAbs and enhanced CoRbs MAb binding to gp120. The CD4bs ligands were VRC01, b12, and CD4-Ig; the CoRbs MAbs were 17b, 48d, and 2.1C. The MAb 2G12 was used as negative control.
Li, Y., O'Dell, S., Wilson, R., Wu, X., Schmidt, S. D., Hogerkorp, C. M.,... & Chakrabarti, B. (2012). HIV-1 Neutralizing Antibodies Display Dual Recognition of the Primary and Coreceptor Binding Sites and Preferential Binding to Fully-Cleaved Envelope Glycoproteins. Journal of virology, JVI-01543.
Figure 2 Binding of VRC03, VRC06, and VRC06b to JR-FL gp160ΔCT-transfected 293T cell surface by FACS assay, represented by MFI (mean of fluorescence intensity). JR-FL gp160ΔCT is the cleaved trimer; JR-FL gp160ΔCT cleavage-defective is the uncleaved trimer, which contains two mutations that render it cleavage defective.
Li, Y., O'Dell, S., Wilson, R., Wu, X., Schmidt, S. D., Hogerkorp, C. M.,... & Chakrabarti, B. (2012). HIV-1 Neutralizing Antibodies Display Dual Recognition of the Primary and Coreceptor Binding Sites and Preferential Binding to Fully-Cleaved Envelope Glycoproteins. Journal of virology, JVI-01543.
Figure 3 VRC06 neutralization potency against JR-FL gp160ΔCT virus was affected by Env mutations leading to an alteration of neutralization sensitivity. Top graphs, Env mutants Δ301 and T569A/I675V display enhanced neutralization sensitivity to CD4bs MAb F105 but substantially decreased neutralization sensitivity to VRC06; the bar graphs on the bottom depict the effects of the Env mutations Δ301 and T569A/I675V on the HIV sensitivity (IC50 values) to VRC06 and other CD4bs ligands.
Li, Y., O'Dell, S., Wilson, R., Wu, X., Schmidt, S. D., Hogerkorp, C. M.,... & Chakrabarti, B. (2012). HIV-1 Neutralizing Antibodies Display Dual Recognition of the Primary and Coreceptor Binding Sites and Preferential Binding to Fully-Cleaved Envelope Glycoproteins. Journal of virology, JVI-01543.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Species Reactivity
- HIV-1
- Clone
- VRC01
- Applications
- WB, ELISA, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for ELISA, FC and Neut. For details, refer to published data.
Target
- Alternative Names
- ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160; precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain
- Gene ID
- 155971
- UniProt ID
- P04578
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Humanized Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-082 | Recombinant Anti-HIV-1 gp120 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| MRO-028LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (MRO-028LC-VHH) | ELISA | Single domain antibody |
| MRO-029LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (MRO-029LC-VHH) | ELISA | Single domain antibody |
| MRO-030LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (MRO-030LC-VHH) | ELISA | Single domain antibody |
| MRO-031LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (MRO-031LC-VHH) | ELISA | Single domain antibody |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-171, RRID: AB_3111633)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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