Recombinant Mouse Anti-HPV-16 E7 Antibody (VC573)

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Q&As

1. What is the preparation protocol for mouse anti-HPV-16 E7 recombinant antibody before beginning an ELISA assay?

   A: Prior to an ELISA assay, dilute the mouse anti-HPV-16 E7 recombinant antibody to the recommended concentration using the appropriate ELISA buffer. Ensure all reagents are at room temperature and mix the solution gently to avoid denaturation. It's essential to follow the protocol specific to the ELISA kit being used.

2. How can mouse anti-HPV-16 E7 recombinant antibody be used to quantify HPV-16 E7 protein in tissue samples?

   A: The antibody can be used in quantitative assays such as ELISA or immunohistochemistry (IHC). For ELISA, prepare tissue lysates and follow the assay protocol to quantify the protein. In IHC, follow the staining protocol to visualize and measure protein levels using imaging software.

3. What is the best method to optimize the concentration of mouse anti-HPV-16 E7 recombinant antibody for Western blot?

   A: Perform a serial dilution of the antibody and test each dilution on a Western blot with positive control samples. Analyze the blots to determine the dilution that provides the best signal-to-noise ratio. Adjust the dilution according to the specific needs of the experiment.

4. How can mouse anti-HPV-16 E7 recombinant antibody be used in immunoprecipitation (IP) experiments?

   A: For IP, incubate the antibody with cell lysates containing the HPV-16 E7 protein. Use protein A/G agarose beads to capture the antibody-protein complex. Wash the beads to remove non-specific binding and elute the protein for analysis by Western blot or mass spectrometry.

5. What are the key considerations when using mouse anti-HPV-16 E7 recombinant antibody in multiplex assays?

   A: When using the antibody in multiplex assays, ensure it is compatible with other antibodies and detection systems used in the assay. Validate the antibody's performance in the multiplex format and optimize concentrations to prevent cross-reactivity and interference.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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