Recombinant Mouse anti-Human DOT1L Monoclonal antibody (EML1954) (CAT#: MOB-1042CT)
Recombinant Mouse monoclonal antibody to Human DOT1L, expressed in Chinese Hamster Ovary cells(CHO).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11875/1201_C5_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11875/1201_C5_1_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71217/1400_C9_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71217/1400_C9_1_selected.jpg
Figure 3 IHC staining of human testis
(Immunohistochemical staining of human testis shows moderate positivity in subset of cells in seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/74977/166292_A_6_6_selected.jpg
(Immunohistochemical staining of human testis shows moderate positivity in subset of cells in seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/74977/166292_A_6_6_selected.jpg
Figure 4 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/74977/166292_B_9_5.jpg
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/74977/166292_B_9_5.jpg
Figure 5 Kidney
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/74977/166292_A_7_5.jpg
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/74977/166292_A_7_5.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104885-DOT1L
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104885-DOT1L
Specifications
- Immunogen
- Recombinant fragment, corresponding to amino acids 3-109 of Human Dot1L
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- EML1954
- Applications
- Used for immunoassay techniques such as: Flow Cytometry; Immunoprecipitation; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- 0.5 mg/ml
- Buffer
- Preservative: None PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- DOT1L; DOT1-like histone H3K79 methyltransferase; DOT1; KMT4; histone-lysine N-methyltransferase, H3 lysine-79 specific; H3-K79-HMTase; DOT1-like protein; lysine N-methyltransferase 4; histone methyltransferase DOT1L; histone H3-K79 methyltransferase; DOT1-like, histone H3 methyltransferase;
- Gene ID
- 84444
- UniProt ID
- Q8TEK3
- Protein Refseq
- NP_115871
- Function
- DNA binding; histone-lysine N-methyltransferase activity; methyltransferase activity; protein binding; transferase activity;
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1037 | Hi-Affi™ Rabbit Anti-DOT1L Recombinant Antibody (clone DS1037AB) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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