Recombinant Rabbit Anti-DOT1L Antibody (clone R01-7F6) (CAT#: VS3-FY461)

Name: Recombinant Rabbit Anti-DOT1L Antibody (clone R01-7F6)
SKU: VS3-FY461
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes human and mouse DOT1L protein. It can detect a band of approximately 165 kDa (observedd molecular weight: 185 kDa).

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

IHC

Figure 1. Recombinant Rabbit Anti-DOT1L Antibody (clone R01-7F6) in IHC.

Figure 1. Recombinant Rabbit Anti-DOT1L Antibody (clone R01-7F6) in IHC.

Immunohistochemical analysis of paraffin-embedded mouse testis using KMT4 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11875/1201_C5_1_selected.jpg

Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71217/1400_C9_1_selected.jpg

Normal Tissue

Figure 3 IHC staining of human testis
(Immunohistochemical staining of human testis shows moderate positivity in subset of cells in seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/74977/166292_A_6_6_selected.jpg

Figure 4 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/74977/166292_B_9_5.jpg

Figure 5 Kidney
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/74977/166292_A_7_5.jpg

RNA Expression

Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104885-DOT1L

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Recombinant protein of human KMT4/Dot1L

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human and Mouse DOT1L

Species Reactivity
Human, Mouse

Clone
R01-7F6

Applications
Western Blot, Immunohistochemistry-Paraffin

Conjugate
Unconjugated

MW
Calculated MW: 165 kDa; Observed MW: 185 kDa

Product Property

Purification
Affinity Purified

Purity
>95%

Format
Liquid

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100

Target

Alternative Names
DOT1 Like Histone Lysine Methyltransferase; Histone H3-K79 Methyltransferase; Histone Methyltransferase DOT1L; Lysine N-Methyltransferase 4; DOT1-Like Protein; H3-K79-HMTase; KMT4; Histone-Lysine N-Methyltransferase, H3 Lysine-79 Specific

Gene ID
84444

UniProt ID
Q8TEK3

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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IgG Antibody Products Rabbit Monoclonal Antibody Products

CAT	Product Name	Application	Type
MOB-1042CT	Recombinant Mouse anti-Human DOT1L Monoclonal antibody (EML1954)	FC, IP, WB
ZG-0639J	Rabbit Anti-DOT1L Recombinant Antibody (clone 3B6)	ELISA, WB	Rabbit IgG

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