Recombinant Rabbit Anti-DOT1L Antibody (clone R01-7F6)
CAT#: VS3-FY461
This product is a recombinant rabbit antibody that recognizes human and mouse DOT1L protein. It can detect a band of approximately 165 kDa (observedd molecular weight: 185 kDa).
Tested Data
Gene Expression
IHC
Figure 1. Recombinant Rabbit Anti-DOT1L Antibody (clone R01-7F6) in IHC.
Immunohistochemical analysis of paraffin-embedded mouse testis using KMT4 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11875/1201_C5_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71217/1400_C9_1_selected.jpg
Normal Tissue
Figure 3 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate positivity in subset of cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74977/166292_A_6_6_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74977/166292_B_9_5.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74977/166292_A_7_5.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104885-DOT1L
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Specifications
- Immunogen
- Recombinant protein of human KMT4/Dot1L
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse DOT1L
- Species Reactivity
- Human, Mouse
- Clone
- R01-7F6
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 165 kDa; Observed MW: 185 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "DOT1L"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1042CT | Recombinant Mouse anti-Human DOT1L Monoclonal antibody (EML1954) | FC, IP, WB | |
| ZG-0639J | Rabbit Anti-DOT1L Recombinant Antibody (clone 3B6) | ELISA, WB | Rabbit IgG |
| VS-0625-FY1452 | Human Anti-DOT1L Recombinant Antibody (clone 2506W1347) | ELISA, IP MS | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1037 | Hi-Affi™ Rabbit Anti-DOT1L Recombinant Antibody (clone DS1037AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2057 | Anti-DOT1L Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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