Anti-Human FCGRT Recombinant Antibody (TAB-450CQ) (CAT#: TAB-450CQ)
Recombinant monoclonal antibody to FCGRT. This antibody is a humanized monoclonal antibody. It is designed to target the neonatal Fc receptor (FcRn), a receptor that plays a crucial role in regulating the half-life and recycling of immunoglobulin G (IgG) antibodies.
Specific Inquiry
WB
Figure 1 Anti-Human FCGRT Antibody (TAB-450CQ) in WB
Western blot analysis of TAB-450CQ was performed by loading Human FCGRT Protein (His Tag) in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-450CQ (Left, 2 μg/mL) and TAB-450CQ-FITC (Right, 2 μg/mL). HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
DB
Figure 2 Anti-Human FCGRT Antibody (TAB-450CQ) in DB
Dot Blot analysis of TAB-450CQ was performed by coating with Human FCGRT Protein (His Tag).
TAB-450CQ (Top) and TAB-450CQ-FITC (Bottom) incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
NC: Negative control PC: Positive control
ELISA
Figure 3 Anti-Human FCGRT Antibody (TAB-450CQ) in ELISA
ELISA analysis of TAB-450CQ was performed by coating with Human FCGRT Protein (His Tag). Then blocked with BSA and incubated with TAB-450CQ and TAB-450CQ-FITC. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 8000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Humanized and chimeric
- Type
- IgG4, κ
- Specificity
- Human FCGRT
- Species Reactivity
- Human
- Applications
- Used for immunoassay techniques such as: Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; Immunofluorescence; Immunoprecipitation; Flow Cytometry; Functional Studies
- Conjugate
- Unconjugated
- Related Disease
- Immunological diseases, Thrombocytopenia
Product Property
- Purity
- > 95% as determined by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Target
Related Resources
Please refer to Rozanolixizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Rozanolixizumab.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0418ZL | Mouse Anti-FCGRT Recombinant Antibody (clone YC053) | FC, ELISA | |
| HPAB-0199-CN | Human Anti-FCGRT Recombinant Antibody (HPAB-0199-CN) | FC | Humanized IgG |
| HPAB-0200-CN | Human Anti-FCGRT Recombinant Antibody (HPAB-0200-CN) | ELISA, FC | Humanized IgG4, κ |
| HPAB-0201-CN | Human Anti-FCGRT Recombinant Antibody (HPAB-0201-CN) | ELISA, FC | Humanized IgG4 |
| HPAB-1853-FY | Human Anti-FCGRT Recombinant Antibody (clone 1D10) | ELISA | Human IgG1 |
Customer Reviews and Q&As
Customer Review
Q&As
Excellent for Flow Cytometry
This antibody works excellently in flow cytometry applications. We used it to assess FCGRT expression on different cell types, and the results were highly reliable and reproducible. The signal was strong, and nonspecific binding was minimal. The availability of custom conjugation services made it even more convenient for our multicolor panels.
Versatile Application
We've successfully used this antibody in various applications, including flow cytometry, immunohistochemistry, and immunofluorescence and immunoprecipitation. Its versatility has allowed us to explore different aspects of FCGRT function without needing multiple antibodies. This has streamlined our workflow and ensured consistent results across different experimental setups.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-450CQ, RRID: AB_3111951)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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