Recombinant Rabbit Anti-MYH9 Antibody (clone R08-2C2)
CAT#: VS3-FY993
This product is a recombinant rabbit antibody that recognizes MYH9. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R08-2C2 is specific for human, rat MYH9. The antigen is synthetic peptide of human non-muscle myosin iia.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & actin filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1644/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies, plasma membrane & actin filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/1685_C7_1_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies, plasma membrane & actin filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1644/62_G1_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_6_6.jpg
Normal Tissue
Figure 8 Endometrium
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_B_6_3.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100345-MYH9
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Specifications
- Immunogen
- Synthetic peptide of human non-muscle myosin IIA.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat MYH9
- Species Reactivity
- Human, Rat
- Clone
- R08-2C2
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 227 kDa; Observed MW: 227 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Myosin Heavy Chain 9; Cellular Myosin Heavy Chain, Type A; Nonmuscle Myosin Heavy Chain II-A; Non-Muscle Myosin Heavy Chain IIa; Non-Muscle Myosin Heavy Chain A; NMMHC-IIA; Myosin, Heavy Polypeptide 9, Non-Muscle; Non-Muscle Myosin Heavy Polypeptide 9; Myosin Heavy Chain, Non-Muscle IIa; Myosin, Heavy Chain 9, Non-Muscle; Non-Muscle Myosin Heavy Chain 9
- Gene ID
- 4627
- UniProt ID
- P35579
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "Myh9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-999 | Recombinant Human Anti-Rat Myh9 Antibody,clone SF9 | ELISA, ICC, WB | IgG |
| MOB-0795CT | Recombinant Mouse anti-Human MYH9 Monoclonal antibody (EML521) | FC, ICC, IF, WB | |
| HPAB-1997-FY | Human Anti-MYH9 Recombinant Antibody (clone CLL412) | IP | Human IgG1 |
| HPAB-1998-FY | Human Anti-MYH9 Recombinant Antibody (clone CLL068) | IP, IHC, IF | Human IgG1 |
| VS-0323-XY20 | Mouse Anti-MYH9 Recombinant Antibody (clone 2-5G2) | ELISA, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2341 | Hi-Affi™ Recombinant Rabbit Anti-MYH9 Monoclonal Antibody (DS2341AB) | WB, IHC, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1997-FY-F(E) | Human Anti-MYH9 Recombinant Antibody (clone CLL412); Fab Fragment | IP | Human Fab |
| HPAB-1998-FY-F(E) | Human Anti-MYH9 Recombinant Antibody (clone CLL068); Fab Fragment | IP, IHC, IF | Human Fab |
| HPAB-1999-FY-F(E) | Human Anti-MYH9 Recombinant Antibody (clone CLL258); Fab Fragment | IP, IHC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1997-FY-S(P) | Human Anti-MYH9 Recombinant Antibody (clone CLL412); scFv Fragment | IP | Human scFv |
| HPAB-1998-FY-S(P) | Human Anti-MYH9 Recombinant Antibody (clone CLL068); scFv Fragment | IP, IHC, IF | Human scFv |
| HPAB-1999-FY-S(P) | Human Anti-MYH9 Recombinant Antibody (clone CLL258); scFv Fragment | IP, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1415 | Anti-MYH9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4636 | Anti-Mouse MYH9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4635 | Anti-Human MYH9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4637 | Anti-Canine MYH9 Immunohistochemistry Kit | IHC |
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