Recombinant Rabbit Anti-MYH9 Antibody (clone R08-2C2) (CAT#: VS3-FY993)
This product is a recombinant rabbit antibody that recognizes MYH9. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R08-2C2 is specific for human, rat MYH9. The antigen is synthetic peptide of human non-muscle myosin iia.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & actin filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1644/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & actin filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1644/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies, plasma membrane & actin filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/1685_C7_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies, plasma membrane & actin filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/1685_C7_1_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies, plasma membrane & actin filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1644/62_G1_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies, plasma membrane & actin filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1644/62_G1_1_blue_red_green.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_B_7_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_B_7_5.jpg
Figure 5 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_8_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_8_3.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_8_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_8_5.jpg
Figure 7 Testis
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_6_6.jpg
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_6_6.jpg
Figure 8 Endometrium
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_B_6_3.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_B_6_3.jpg
Figure 9 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64783/148113_A_8_8.jpg
Figure 10 RNA cell line category: Cell line enhanced (TIME)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100345-MYH9
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100345-MYH9
Figure 10 RNA cell line category: Cell line enhanced (TIME)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100345-MYH9
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100345-MYH9
Specifications
- Immunogen
- Synthetic peptide of human non-muscle myosin IIA.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat MYH9
- Species Reactivity
- Human, Rat
- Clone
- R08-2C2
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 227 kDa; Observed MW: 227 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Myosin Heavy Chain 9; Cellular Myosin Heavy Chain, Type A; Nonmuscle Myosin Heavy Chain II-A; Non-Muscle Myosin Heavy Chain IIa; Non-Muscle Myosin Heavy Chain A; NMMHC-IIA; Myosin, Heavy Polypeptide 9, Non-Muscle; Non-Muscle Myosin Heavy Polypeptide 9; Myosin Heavy Chain, Non-Muscle IIa; Myosin, Heavy Chain 9, Non-Muscle; Non-Muscle Myosin Heavy Chain 9
- Gene ID
- 4627
- UniProt ID
- P35579
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-999 | Recombinant Human Anti-Rat Myh9 Antibody,clone SF9 | ELISA, ICC, WB | IgG |
| MOB-0795CT | Recombinant Mouse anti-Human MYH9 Monoclonal antibody (EML521) | FC, ICC/IF, WB | |
| HPAB-1997-FY | Human Anti-MYH9 Recombinant Antibody (clone CLL412) | IP | Human IgG1 |
| HPAB-1998-FY | Human Anti-MYH9 Recombinant Antibody (clone CLL068) | IP, IHC, IF | Human IgG1 |
| HPAB-1999-FY | Human Anti-MYH9 Recombinant Antibody (clone CLL258) | IP, IHC | Human IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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