Recombinant Mouse anti-Human NR3C2 Monoclonal antibody (FQS6265)
CAT#: MOB-2382CT
Recombinant Mouse Monoclonall antibody to Human NR3C2, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74979/1745_B6_7_cr5806280f507ab_selected.jpg
Normal Tissue
Figure 2 Rectum.
High expression in rectum
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9155/31446_A_5_3_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9155/31360_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9155/31446_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9155/31446_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9155/31446_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9155/31446_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9155/31446_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (hTERT-RPE1, HUVEC TERT2, Karpas-707, PC-3, RPTEC TERT1, TIME)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000151623-NR3C2
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Specifications
- Immunogen
- Baculovirus-expressed recombinant fragment: ETKGYHSLPE GLDMERRWGQ VSQAVERSSL GPTERTDENN YMEIVNVSCV SGAIPNNSTQ GSSKEKQELL PCLQQDNNRP GILTSDIKTE LESKELSA, corresponding to amino acids 2-99 of Human Mineralocorticoid Receptor.
- Host Species
- Mouse
- Specificity
- Ubiquitous. Highly expressed in distal tubules, convoluted tubules and cortical collecting duct in kidney, and in sweat glands. Detected at lower levels in cardiomyocytes, in epidermis and in colon enterocytes.
- Species Reactivity
- Human
- Clone
- FQS6265
- Applications
- Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Immunoprecipitation; Immunohistochemistry paraffin embedded sections; Western Blot; Flow Cytometry; Immunocytochemistry/Immunofluorescence
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Buffer
- Physiological Saline. Preservative: 0.1% Sodium Azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- NR3C2; nuclear receptor subfamily 3, group C, member 2; MR; MCR; MLR; NR3C2VIT; mineralocorticoid receptor; aldosterone receptor; mineralocorticoid receptor 1; mineralocorticoid receptor delta;
- Gene ID
- 4306
- UniProt ID
- P08235
- Protein Refseq
- NP_000892
- Function
- metal ion binding; protein binding; receptor activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; steroid binding; steroid hormone receptor activity; zinc ion binding;
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MOB-2155z | Mouse Anti-NR3C2 Recombinant Antibody (clone 35A10) | WB, FC, ICC, IF, IHC, Neut | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| NEUT-1789CQ | Recombinant Mouse Anti-NR3C2 Antibody (H10E4C9F) | WB, Neut, Flow, ICC, IF, IHC, IHC-P | IgG1 |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1515 | Anti-NR3C2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4962 | Anti-Mouse NR3C2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4961 | Anti-Human NR3C2 Immunohistochemistry Kit | IHC |
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