Recombinant Mouse anti-Human SMURF1 Monoclonal antibody (EML1749) (CAT#: MOB-1894CT)
Recombinant Mouse monoclonal antibody to Human SMURF1, expressed in Chinese Hamster Ovary cells(CHO).
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1924_A2_3_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1924_A2_3_selected.jpg
Figure 2 IHC staining of human esophagus
(Immunohistochemical staining of human esophagus shows membrane positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_B_6_1_selected.jpg
(Immunohistochemical staining of human esophagus shows membrane positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_B_6_1_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1841_E2_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1841_E2_1_blue_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1899_D1_5_cr5ba0db0f311d8_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1899_D1_5_cr5ba0db0f311d8_blue_red_green.jpg
Figure 5 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_B_7_5.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_B_7_5.jpg
Figure 6 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_8_3.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_8_3.jpg
Figure 7 Liver
(Cholangiocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_9_4.jpg
(Cholangiocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_9_4.jpg
Figure 8 Kidney
(Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_9_5.jpg
(Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_9_5.jpg
Figure 9 RNA cell line category: Cell line enhanced (A-431, BJ, EFO-21, HDLM-2, PC-3, U-2 OS, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198742-SMURF1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198742-SMURF1
Specifications
- Immunogen
- Recombinant fragment, corresponding to amino acids 165-269 of Human SMURF1
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- EML1749
- Applications
- Used for immunoassay techniques such as: Immunocytochemistry/Immunofluorescence; Immunohistochemistry paraffin embedded sections; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- Batch dependent within range:0.5 -1.0 mg/ml
- Buffer
- Preservative: None PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- SMURF1; SMAD specific E3 ubiquitin protein ligase 1; E3 ubiquitin-protein ligase SMURF1; hSMURF1; E3 ubiquitin ligase SMURF1; Smad-specific E3 ubiquitin ligase 1; Smad ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1;
- Gene ID
- 57154
- UniProt ID
- Q9HCE7
- Protein Refseq
- NP_001186776
- Function
- I-SMAD binding; R-SMAD binding; acid-amino acid ligase activity; activin binding; ligase activity; protein binding; ubiquitin-protein ligase activity;
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
Fab Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-553 | Recombinant Human Anti-Human SMURF1 Antibody, clone D560 | IP, IF, ELISA | IgG |
| VS4-CJ122 | Recombinant Mouse Anti-SMURF1 Antibody (clone 1A9H4) | ELISA, WB, IHC, IF, FC | Mouse IgG2a |
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