Recombinant Human Anti-Human SMURF1 Antibody, clone D560 (CAT#: DrMAB-553)
Recombinant Human Antibody binds selectively to Human SMURF1, expressed in E. coli.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1924_A2_3_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1924_A2_3_selected.jpg
Figure 2 IHC staining of human esophagus
(Immunohistochemical staining of human esophagus shows membrane positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_B_6_1_selected.jpg
(Immunohistochemical staining of human esophagus shows membrane positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_B_6_1_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1841_E2_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1841_E2_1_blue_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1899_D1_5_cr5ba0db0f311d8_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1899_D1_5_cr5ba0db0f311d8_blue_red_green.jpg
Figure 5 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_B_7_5.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_B_7_5.jpg
Figure 6 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_8_3.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_8_3.jpg
Figure 7 Liver
(Cholangiocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_9_4.jpg
(Cholangiocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_9_4.jpg
Figure 8 Kidney
(Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_9_5.jpg
(Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_9_5.jpg
Figure 9 RNA cell line category: Cell line enhanced (A-431, BJ, EFO-21, HDLM-2, PC-3, U-2 OS, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198742-SMURF1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198742-SMURF1
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Type
- IgG
- Specificity
- reacts with human SMURF1
- Species Reactivity
- Human
- Clone
- D560
- Applications
- IP; IF; ELISA
Product Property
- Storage
- short term – store at 4°C (over 6 months), long term - PBS -20°C or -80°C
Target
- Alternative Names
- SMURF1; SMAD specific E3 ubiquitin protein ligase 1; E3 ubiquitin-protein ligase SMURF1; E3 ubiquitin ligase SMURF1; SMAD-specific E3 ubiquitin-protein ligase 1; Smad ubiquitination regulatory factor 1; Smad-specific E3 ubiquitin ligase 1; hSMURF1
- Gene ID
- 57154
- UniProt ID
- Q9HCE7
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Antibody Products
IgG Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-554 | Recombinant Human Anti-Human SMURF1 Antibody Fab Fragment, clone D562 | IP, IF, ELISA, IP-MS | Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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