Recombinant Human Anti-Human SMURF1 Antibody, clone D560
CAT#: DrMAB-553
Recombinant Human Antibody binds selectively to Human SMURF1, expressed in E. coli.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1924_A2_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows membrane positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_B_6_1_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1841_E2_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1899_D1_5_cr5ba0db0f311d8_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_8_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_9_5.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (A-431, BJ, EFO-21, HDLM-2, PC-3, U-2 OS, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198742-SMURF1
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Type
- IgG
- Specificity
- reacts with human SMURF1
- Species Reactivity
- Human
- Clone
- D560
- Applications
- IP; IF; ELISA
Product Property
- Storage
- short term – store at 4°C (over 6 months), long term - PBS -20°C or -80°C
Target
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SMURF1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-554 | Recombinant Human Anti-Human SMURF1 Antibody Fab Fragment, clone D562 | IP, IF, ELISA, IP-MS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1894CT | Recombinant Mouse anti-Human SMURF1 Monoclonal antibody (EML1749) | ICC, IF, IHC-P, WB | |
| VS4-CJ122 | Recombinant Mouse Anti-SMURF1 Antibody (clone 1A9H4) | ELISA, WB, IHC, IF, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY241 | AbPlus™ Anti-SMURF1 Magnetic Beads (D560) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6760 | Anti-SMURF1 Immunohistochemistry Kit | IHC |
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