Recombinant Mouse Anti-SMURF1 Antibody (clone 1A9H4)
CAT#: VS4-CJ122
This mouse anti-SMURF1 antibody (clone 1A9H4) is a product that recognizes human SMURF1. The immunogen of this antibody is recombinant human E3 ubiquitin-protein ligase SMURF1 protein (198aa-374aa). This product was purified by protein G purified and it has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1924_A2_3_selected.jpg
❮
❯
Specifications
- Immunogen
- Recombinant Human E3 ubiquitin-protein ligase SMURF1 protein (198aa-374aa)
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human SMURF1
- Species Reactivity
- Human
- Clone
- 1A9H4
- Applications
- ELISA, WB, IHC, IF, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, PBS, pH 7.3.
- Preservative
- 0.1% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).
Target
- Alternative Names
- SMAD specific E3 ubiquitin protein ligase 1
- Gene ID
- 57154
- UniProt ID
- Q9HCE7
- Long Name
- SMAD specific E3 ubiquitin protein ligase 1
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane
- Post Translation Modifications
- Auto-ubiquitinated in presence of NDFIP1 (PubMed:23087404).
Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
- Protein Refseq
- NP_001186776.1; NP_065162.1; NP_851994.1
- Function
- E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404).
Acts as an antagonist of TGF-beta signaling by ubiquitinating TGFBR1 and targeting it for degradation (PubMed:21791611).
Plays a role in dendrite formation by melanocytes (PubMed:23999003).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS4-CJ122. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "SMURF1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-553 | Recombinant Human Anti-Human SMURF1 Antibody, clone D560 | IP, IF, ELISA | IgG |
| MOB-1894CT | Recombinant Mouse anti-Human SMURF1 Monoclonal antibody (EML1749) | ICC, IF, IHC-P, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-554 | Recombinant Human Anti-Human SMURF1 Antibody Fab Fragment, clone D562 | IP, IF, ELISA, IP-MS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY241 | AbPlus™ Anti-SMURF1 Magnetic Beads (D560) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6760 | Anti-SMURF1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, Neut
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, IHC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: WB, IF, FuncS
Application: ELISA, FuncS, IHC, IF, FC, ADCC
Application: ELISA, Neut
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, IHC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
