Recombinant Mouse Anti-SMURF1 Antibody (clone 1A9H4) (CAT#: VS4-CJ122)

This mouse anti-SMURF1 antibody (clone 1A9H4) is a product that recognizes human SMURF1. The immunogen of this antibody is recombinant human E3 ubiquitin-protein ligase SMURF1 protein (198aa-374aa). This product was purified by protein G purified and it has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).

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Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression

Specifications

  • Immunogen
  • Recombinant Human E3 ubiquitin-protein ligase SMURF1 protein (198aa-374aa)
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2a
  • Specificity
  • Human SMURF1
  • Species Reactivity
  • Human
  • Clone
  • 1A9H4
  • Applications
  • ELISA, WB, IHC, IF, FC
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Protein G purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • 50% Glycerol, PBS, pH 7.3.
  • Preservative
  • 0.1% Sodium Azide
  • Storage
  • Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

Applications

  • Application Notes
  • This antibody has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).

Target

  • Alternative Names
  • SMAD specific E3 ubiquitin protein ligase 1
  • Long Name
  • SMAD specific E3 ubiquitin protein ligase 1
  • Cellular Localization
  • Cell membrane, Cytoplasm, Membrane
  • Post Translation Modifications
  • Auto-ubiquitinated in presence of NDFIP1 (PubMed:23087404).
    Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
  • Function
  • E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404).
    Acts as an antagonist of TGF-beta signaling by ubiquitinating TGFBR1 and targeting it for degradation (PubMed:21791611).
    Plays a role in dendrite formation by melanocytes (PubMed:23999003).

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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