Recombinant Mouse Anti-SMURF1 Antibody (clone 1A9H4) (CAT#: VS4-CJ122)
This mouse anti-SMURF1 antibody (clone 1A9H4) is a product that recognizes human SMURF1. The immunogen of this antibody is recombinant human E3 ubiquitin-protein ligase SMURF1 protein (198aa-374aa). This product was purified by protein G purified and it has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1924_A2_3_selected.jpg

(Immunohistochemical staining of human esophagus shows membrane positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_B_6_1_selected.jpg

(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1841_E2_1_blue_red_green.jpg

(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1899_D1_5_cr5ba0db0f311d8_blue_red_green.jpg

(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_B_7_5.jpg

(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_8_3.jpg

(Cholangiocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_9_4.jpg

(Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_9_5.jpg

(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198742-SMURF1
Specifications
- Immunogen
- Recombinant Human E3 ubiquitin-protein ligase SMURF1 protein (198aa-374aa)
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human SMURF1
- Species Reactivity
- Human
- Clone
- 1A9H4
- Applications
- ELISA, WB, IHC, IF, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, PBS, pH 7.3.
- Preservative
- 0.1% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).
Target
- Alternative Names
- SMAD specific E3 ubiquitin protein ligase 1
- Gene ID
- 57154
- UniProt ID
- Q9HCE7
- Long Name
- SMAD specific E3 ubiquitin protein ligase 1
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane
- Post Translation Modifications
- Auto-ubiquitinated in presence of NDFIP1 (PubMed:23087404).
Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
- Protein Refseq
- NP_001186776.1; NP_065162.1; NP_851994.1
- Function
- E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404).
Acts as an antagonist of TGF-beta signaling by ubiquitinating TGFBR1 and targeting it for degradation (PubMed:21791611).
Plays a role in dendrite formation by melanocytes (PubMed:23999003).
Recommended Products
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "SMURF1"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
DrMAB-553 | Recombinant Human Anti-Human SMURF1 Antibody, clone D560 | IP, IF, ELISA | IgG |
MOB-1894CT | Recombinant Mouse anti-Human SMURF1 Monoclonal antibody (EML1749) | ICC/IF, IHC-P, WB |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS4-CJ122. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: FC, Cyt, Stim, PP, Agonist
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: Inhib, Cyt
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: Block, IP, IF, FC
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: Neut, ELISA, WB, SPR, IF, EM
Application: Neut, IHC, Activ, FuncS, IF, ELISA
Application: ELISA
Application: ELISA, IHC, WB
Application: IB, FC, ELISA, FuncS
For Research Use Only. Not For Clinical Use.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.