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    Recombinant Mouse Anti-SMURF1 Antibody (clone 1A9H4) (CAT#: VS4-CJ122)

    This mouse anti-SMURF1 antibody (clone 1A9H4) is a product that recognizes human SMURF1. The immunogen of this antibody is recombinant human E3 ubiquitin-protein ligase SMURF1 protein (198aa-374aa). This product was purified by protein G purified and it has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).

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    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1924_A2_3_selected.jpg
    Figure 2 IHC staining of human esophagus
    Figure 2 IHC staining of human esophagus
    (Immunohistochemical staining of human esophagus shows membrane positivity in squamous epithelial cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_B_6_1_selected.jpg
    Figure 3 IF staining of human cell line U-2 OS
    Figure 3 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1841_E2_1_blue_red_green.jpg
    Figure 4 IF staining of human cell line U-251 MG
    Figure 4 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19671/1899_D1_5_cr5ba0db0f311d8_blue_red_green.jpg
    Normal Tissue
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glial cells
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_B_7_5.jpg
    Figure 6 Colon
    Figure 6 Colon
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glandular cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_8_3.jpg
    Figure 7 Liver
    Figure 7 Liver
    (Cholangiocytes
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_9_4.jpg
    Figure 8 Kidney
    Figure 8 Kidney
    (Cells in tubules
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55245/155345_A_9_5.jpg
    RNA Expression
    Figure 9 RNA cell line category: Cell line enhanced (A-431, BJ, EFO-21, HDLM-2, PC-3, U-2 OS, U-251 MG)
    Figure 9 RNA cell line category: Cell line enhanced (A-431, BJ, EFO-21, HDLM-2, PC-3, U-2 OS, U-251 MG)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198742-SMURF1

    Specifications

    • Immunogen
    • Recombinant Human E3 ubiquitin-protein ligase SMURF1 protein (198aa-374aa)
    • Host Species
    • Mouse
    • Type
    • Mouse IgG2a
    • Specificity
    • Human SMURF1
    • Species Reactivity
    • Human
    • Clone
    • 1A9H4
    • Applications
    • ELISA, WB, IHC, IF, FC
    • Conjugate
    • Unconjugated

    Product Property

    • Purification
    • Protein G purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Format
    • Liquid
    • Buffer
    • 50% Glycerol, PBS, pH 7.3.
    • Preservative
    • 0.1% Sodium Azide
    • Storage
    • Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

    Applications

    • Application Notes
    • This antibody has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).

    Target

    • Alternative Names
    • SMAD specific E3 ubiquitin protein ligase 1
    • Long Name
    • SMAD specific E3 ubiquitin protein ligase 1
    • Cellular Localization
    • Cell membrane, Cytoplasm, Membrane
    • Post Translation Modifications
    • Auto-ubiquitinated in presence of NDFIP1 (PubMed:23087404).
      Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
    • Function
    • E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404).
      Acts as an antagonist of TGF-beta signaling by ubiquitinating TGFBR1 and targeting it for degradation (PubMed:21791611).
      Plays a role in dendrite formation by melanocytes (PubMed:23999003).

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    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

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    See other products for "SMURF1"

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    DrMAB-553 Recombinant Human Anti-Human SMURF1 Antibody, clone D560 IP, IF, ELISA IgG
    MOB-1894CT Recombinant Mouse anti-Human SMURF1 Monoclonal antibody (EML1749) ICC/IF, IHC-P, WB

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