Mouse Anti-IFNB1 Recombinant Antibody (clone B-02) (CAT#: NEUT-987CQ)

Figure 1 Mouse Anti-IFNB1 Recombinant Antibody (NEUT-987CQ) in SDS-PAGE

SDS-PAGE analysis of NEUT-987CQ in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 275 kDa.

Figure 2 Mouse Anti-IFNB1 Recombinant Antibody (NEUT-987CQ) in SEC-HPLC

SEC-HPLC analysis of NEUT-987CQ shows that the purity is more than 90%.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Mouse Anti-IFNB1 Recombinant Antibody (NEUT-987CQ) in DB

Dot Blot analysis of NEUT-987CQ was performed by coating with human IFN-beta protein (His tag).

NEUT-987CQ incubation concentration: 2 μg/mL.

HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)

Figure 4 Mouse Anti-IFNB1 Recombinant Antibody (NEUT-987CQ) in WB

Western blot analysis of NEUT-987CQ was performed by loading human IFN-beta protein, His tag.

NEUT-987CQ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L).

Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 2: Reducing Antigen (0.6 μg)

Figure 5 Mouse Anti-IFNB1 Recombinant Antibody (NEUT-987CQ) in ELISA

ELISA analysis of NEUT-987CQ was performed by coating with human IFN-beta protein (His tag). Then blocked with BSA and incubated with NEUT-987CQ. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.