Anti-Human LCN2 Recombinant Antibody (1-903-430) (CAT#: TAB-487MZ)

Figure 1 is a graph that shows that purified antibody from subclone 1-903-430 demonstrates a similar dose response curve with biotin labeled human NGAL antigen (NGAL-Bt) when compared to parental mAb 1-903-102, thereby illustrating that the subcloning process did not alter the subclones' functional performance.

Symbols: (- ♦ -) mAb 1-903-102 with NGAL-Bt; (- • -) mAb 1-903-430 with NGAL-Bt; (-■-) mAb 1-903-102 with irrelevant biotinylated antigen (NC Ag-Bt); (- x - ) mAb 1-903- 430 with NC Ag-Bt.

Figure 2 is an isotherm binding data graph for monoclonal antibody 1-903-430 binding to different functional epitopes .

Symbols:(- o - , solid line) wild-type NGAL epitope; (- π - , solid line) NGAL epitope comprising S14A mutation; (- 0 - , long dashes line) NGAL epitope comprising K15A mutation; (- x - , short dashes line) NGAL epitope comprising R109A mutation; (- + - , dotted line) NGAL epitope comprising S 158 A mutation; (- Δ - , extra long dashes line) NGAL epitope comprising L159A mutation; (- • -) NGAL epitope comprising G160A mutation.

Figure 3 shows the binding curves of anti-NGAL mAbs and NGAL (free NGAL concentration on the abscissa and fraction bound on the ordinate), and their calculated dissociation constants (Ka).

Symbols: (-•- ) mAb 903; (-■-) mAb 809; (-o- ) mAb 2322; (- T- ) mAb 181; (- Δ - ) mAb 269.