Recombinant Human Antibody (BHA10) is capable of binding to LTBR, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-LTBR mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-LTBR proteins mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Figure 1 Antigen binding of E09-IgG1, BHA10-IgG1, G28.5-IgG1 and their GpL(CT-LC) variants.
HEK293 cells and CD95 or LTβR expressing HEK293 transfectants and HT1080 cells and HT1080-CD40 transfectants were seeded in the upper and lower half of a 24-well tissue culture plate and were pairwise incubated the next day (1.5 h, 37°C) with the indicated concentrations of GpL(CT-LC)-E09-IgG1, GpL(CT-LC)-BHA10-IgG1 and GpL(CT-LC)-G28.5-IgG1. Finally, cell-associated luciferase activity was measured and specific binding values for the GpL-IgG1 fusion proteins were obtained by subtracting the non-specific binding values derived of the HEK293 or HT1080 cells from the corresponding receptor transfectants.
Kums, J., Nelke, J., Rüth, B., Schäfer, V., Siegmund, D., & Wajant, H. (2017, April). Quantitative analysis of cell surface antigen-antibody interaction using Gaussia princeps luciferase antibody fusion proteins. In MAbs (Vol. 9, No. 3, pp. 506-520). Taylor & Francis.
Figure 2 Agonistic activity of E09-IgG1, BHA10-IgG1, G28.5-IgG1 and their GpL(CT-LC) variants.
HT1080-CD40 cells which endogenously express CD95 where cocultured with HEK293 cells transiently transfected with the indicated FcγRs (cotransfection of FcϵR1γ in case of CD16 and CD64) and were stimulated overnight in triplicates with 3000, 300, 30 and 3 ng/ml of E09-IgG1 and 1000, 100, 10 and 1 ng/ml G28.5-IgG1 and the corresponding GpL(CT-LC) antibody variants. In the case of E09-IgG1 20 µM ZVAD and 1 µg/ml CHX were added before stimulation. For coculture with BHA10-IgG1 the same experiment was performed with HT29 cells and 3000, 300 and 30 ng/ml of the antibody. IL8 production was finally measured by ELISA analysis of coculture supernatants.
Kums, J., Nelke, J., Rüth, B., Schäfer, V., Siegmund, D., & Wajant, H. (2017, April). Quantitative analysis of cell surface antigen-antibody interaction using Gaussia princeps luciferase antibody fusion proteins. In MAbs (Vol. 9, No. 3, pp. 506-520). Taylor & Francis.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-2179z | Mouse Anti-LTBR Recombinant Antibody (clone 28H4) | WB, FC | Mouse IgG1 |
MOB-1634MZ | Recombinant Mouse Anti-Human LTBR Antibody (clone NN0572-4U35) | FC, WB | Mouse antibody |
FN-022CQ | Recombinant Rat Anti-LTBR Antibody (3C8) | FC, FuncS, WB | Rat IgG1, κ |
FN-024CQ | Recombinant Rat Anti-LTBR Antibody (4H8 WH2) | FC, FuncS | Rat IgG2a |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-504CL | Human Anti-LTBR Recombinant Antibody (TAB-504CL) | ELISA | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-631 | Human Anti-LTBR Recombinant Antibody (clone BHA10); Fab Fragment | WB, IF, FuncS | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-631 | Human Anti-LTBR Recombinant Antibody (clone BHA10); scFv Fragment | WB, IF, FuncS | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0331MZ | Chicken Anti-LTBR Polyclonal IgY | WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-098LC | Recombinant Rat Anti-Mouse Ltbr Agonist Antibody (5G11) | IP | Rat antibody |
MOB-099LC | Recombinant Mouse Anti-Human LTBR Agonist Antibody (CBE11) | ELISA, FunS, WB, IP | Mouse antibody |
MOB-100LC | Recombinant Humanized Anti-Human LTBR Agonist Antibody (huCBE11) | ELISA, FunS, WB, IP | Humanized antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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