Human Anti-LTBR Recombinant Antibody (clone BHA10) (CAT#: PABL-636)
Recombinant Human Antibody (BHA10) is capable of binding to LTBR, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-LTBR mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-LTBR proteins mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
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Figure 1 Antigen binding of E09-IgG1, BHA10-IgG1, G28.5-IgG1 and their GpL(CT-LC) variants.
HEK293 cells and CD95 or LTβR expressing HEK293 transfectants and HT1080 cells and HT1080-CD40 transfectants were seeded in the upper and lower half of a 24-well tissue culture plate and were pairwise incubated the next day (1.5 h, 37°C) with the indicated concentrations of GpL(CT-LC)-E09-IgG1, GpL(CT-LC)-BHA10-IgG1 and GpL(CT-LC)-G28.5-IgG1. Finally, cell-associated luciferase activity was measured and specific binding values for the GpL-IgG1 fusion proteins were obtained by subtracting the non-specific binding values derived of the HEK293 or HT1080 cells from the corresponding receptor transfectants.
Kums, J., Nelke, J., Rüth, B., Schäfer, V., Siegmund, D., & Wajant, H. (2017, April). Quantitative analysis of cell surface antigen-antibody interaction using Gaussia princeps luciferase antibody fusion proteins. In MAbs (Vol. 9, No. 3, pp. 506-520). Taylor & Francis.
Figure 2 Agonistic activity of E09-IgG1, BHA10-IgG1, G28.5-IgG1 and their GpL(CT-LC) variants.
HT1080-CD40 cells which endogenously express CD95 where cocultured with HEK293 cells transiently transfected with the indicated FcγRs (cotransfection of FcϵR1γ in case of CD16 and CD64) and were stimulated overnight in triplicates with 3000, 300, 30 and 3 ng/ml of E09-IgG1 and 1000, 100, 10 and 1 ng/ml G28.5-IgG1 and the corresponding GpL(CT-LC) antibody variants. In the case of E09-IgG1 20 µM ZVAD and 1 µg/ml CHX were added before stimulation. For coculture with BHA10-IgG1 the same experiment was performed with HT29 cells and 3000, 300 and 30 ng/ml of the antibody. IL8 production was finally measured by ELISA analysis of coculture supernatants.
Kums, J., Nelke, J., Rüth, B., Schäfer, V., Siegmund, D., & Wajant, H. (2017, April). Quantitative analysis of cell surface antigen-antibody interaction using Gaussia princeps luciferase antibody fusion proteins. In MAbs (Vol. 9, No. 3, pp. 506-520). Taylor & Francis.
Specifications
- Immunogen
- Human lymphotoxin beta receptor (TNFR superfamily, member 3)
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG
- Specificity
- Human LTBR
- Species Reactivity
- Human
- Clone
- BHA10
- Applications
- WB, IF, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- LTBR; lymphotoxin beta receptor (TNFR superfamily, member 3); CD18; TNFCR; TNFR3; D12S370; TNFR-RP; TNFRSF3; TNFR2-RP; LT-BETA-R; TNF-R-III; tumor necrosis factor receptor superfamily member 3; lymphotoxin B receptor; tumor necrosis factor C receptor; tumor necrosis factor receptor type III; tumor necrosis factor receptor 2-related protein
- Gene ID
- 4055
- UniProt ID
- P36941
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2179z | Mouse Anti-LTBR Recombinant Antibody (clone 28H4) | WB, FC | Mouse IgG1 |
| MOB-1634MZ | Recombinant Mouse Anti-Human LTBR Antibody (clone NN0572-4U35) | FC, WB | Mouse antibody |
| FN-022CQ | Recombinant Rat Anti-LTBR Antibody (3C8) | FC, FuncS, WB | Rat IgG1, κ |
| FN-024CQ | Recombinant Rat Anti-LTBR Antibody (4H8 WH2) | FC, FuncS | Rat IgG2a |
| VS7-0425-WR321 | Mouse Anti-LTBR Recombinant Antibody (clone 1D1C5C8) | WB, FC | Mouse IgG2a |
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Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-636, RRID: AB_3111665)
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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