Human Anti-LTBR Recombinant Antibody (clone BHA10)
CAT#: PABL-636
Recombinant Human Antibody (BHA10) is capable of binding to LTBR, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-LTBR mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-LTBR proteins mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Published Data
Gene Expression
Figure 1 Antigen binding of E09-IgG1, BHA10-IgG1, G28.5-IgG1 and their GpL(CT-LC) variants.
HEK293 cells and CD95 or LTβR expressing HEK293 transfectants and HT1080 cells and HT1080-CD40 transfectants were seeded in the upper and lower half of a 24-well tissue culture plate and were pairwise incubated the next day (1.5 h, 37°C) with the indicated concentrations of GpL(CT-LC)-E09-IgG1, GpL(CT-LC)-BHA10-IgG1 and GpL(CT-LC)-G28.5-IgG1. Finally, cell-associated luciferase activity was measured and specific binding values for the GpL-IgG1 fusion proteins were obtained by subtracting the non-specific binding values derived of the HEK293 or HT1080 cells from the corresponding receptor transfectants.
Kums, J., Nelke, J., Rüth, B., Schäfer, V., Siegmund, D., & Wajant, H. (2017, April). Quantitative analysis of cell surface antigen-antibody interaction using Gaussia princeps luciferase antibody fusion proteins. In MAbs (Vol. 9, No. 3, pp. 506-520). Taylor & Francis.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line
Immunofluorescent staining of human cell line RT4 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61617/1766_D5_3_selected.jpg
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Specifications
- Immunogen
- Human lymphotoxin beta receptor (TNFR superfamily, member 3)
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG
- Specificity
- Human LTBR
- Species Reactivity
- Human
- Clone
- BHA10
- Applications
- WB, IF, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- LTBR; lymphotoxin beta receptor (TNFR superfamily, member 3); CD18; TNFCR; TNFR3; D12S370; TNFR-RP; TNFRSF3; TNFR2-RP; LT-BETA-R; TNF-R-III; tumor necrosis factor receptor superfamily member 3; lymphotoxin B receptor; tumor necrosis factor C receptor; tumor necrosis factor receptor type III; tumor necrosis factor receptor 2-related protein
- Gene ID
- 4055
- UniProt ID
- P36941
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(Creative Biolabs Cat# PABL-636, RRID: AB_3111665)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2179z | Mouse Anti-LTBR Recombinant Antibody (clone 28H4) | WB, FC | Mouse IgG1 |
| MOB-1634MZ | Recombinant Mouse Anti-Human LTBR Antibody (clone NN0572-4U35) | FC, WB | Mouse antibody |
| FN-022CQ | Recombinant Rat Anti-LTBR Antibody (3C8) | FC, FuncS, WB | Rat IgG1, κ |
| FN-024CQ | Recombinant Rat Anti-LTBR Antibody (4H8 WH2) | FC, FuncS | Rat IgG2a |
| VS7-0425-WR321 | Mouse Anti-LTBR Recombinant Antibody (clone 1D1C5C8) | WB, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-504CL | Human Anti-LTBR Recombinant Antibody (TAB-504CL) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-631 | Human Anti-LTBR Recombinant Antibody (clone BHA10); Fab Fragment | WB, IF, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-631 | Human Anti-LTBR Recombinant Antibody (clone BHA10); scFv Fragment | WB, IF, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0331MZ | Chicken Anti-LTBR Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-098LC | Recombinant Rat Anti-Mouse Ltbr Agonist Antibody (5G11) | IP | Rat antibody |
| MOB-099LC | Recombinant Mouse Anti-Human LTBR Agonist Antibody (CBE11) | ELISA, FunS, WB, IP | Mouse antibody |
| MOB-100LC | Recombinant Humanized Anti-Human LTBR Agonist Antibody (huCBE11) | ELISA, FunS, WB, IP | Humanized antibody |
| VS-0724-XY34 | Rat Anti-Ltbr Agonistic Antibody (clone 4H8 WH2) | FC, Functional assays | Rat IgG2a |
| VS-0724-XY35 | Mouse Anti-LTBR Agonistic Antibody (VS-0724-XY35) | Agonistic assays | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC405 | Recombinant Anti-LTBR Vesicular Antibody, EV Displayed (VS-0425-YC405) | ELISA, FC, Cell-uptake |
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