Mouse Anti-LTBR Recombinant Antibody (clone 1D1C5C8)
CAT#: VS7-0425-WR321
This product is a mouse IgG2a antibody that binds to human LTBR. The antibody 1D1C5C8 recognizes specific amino acid domain of human LTBR (AA: extra 31-227). It can be used for WB and FC assays. It cross reacts with mouse.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line
Immunofluorescent staining of human cell line RT4 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61617/1766_D5_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26202/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human spleen
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells of the red pulp.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26202/ihc_selected.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26202/58102_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26202/58102_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26202/58102_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HHSteC)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111321-LTBR
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Specifications
- Immunogen
- Recombinant human LTBR (AA: extra 31-227) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human LTBR
- Species Reactivity
- Human, Mouse
- Clone
- 1D1C5C8
- Molecule Class
- Tumor Necrosis Factor Receptor
- Applications
- WB, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- LTBR; Tumor Necrosis Factor Receptor Type III; TNF-R-III; Lymphotoxin-Beta Receptor; TNFRSF3; TNFCR; Lymphotoxin B Receptor; CD18; TNFR2-RP; D12S370; TNFR-III; Tumor Necrosis Factor Receptor Superfamily Member 3; LT-BETA-R; Tumor Necrosis Factor C Recepto
- Gene ID
- 4055
- UniProt ID
- P36941
- Long Name
- Lymphotoxin beta receptor
- Cellular Localization
- Membrane
- Post Translation Modifications
- Disulfide bond; Glycoprotein; Phosphoprotein
- Protein Refseq
- NP_001257916.1
- Function
- Receptor
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Reconstitution Buffer
- CAT
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Recommended Dilution Buffer
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- Product Name
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| CAT | Product Name | Application | Type |
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