Anti-Human LTK Recombinant Antibody (FAM150B)
CAT#: TAB-0712CL
Recombinant monoclonal antibody FAM150B is a human monoclonal antibody that specifically binds to human LTK and can be potentially used in the treatment of autoimmune diseases and neurodegenerative diseases.
Published Data
Gene Expression
FuncS
Figure 1 Induction of LTK phosphorylation by FAM150B.
Figure 2 mRNA expression of FAM150B in various cell lines.
Figure 3 mRNA expression of FAM150B in a panel of human immune cells.
Activ
Figure 4 FAM150A and FAM150B activate ALK.
Neurite outgrowth in PC12 cells expressing either vector control, FAM150A, FAM150B, ALK, FAM150A and ALK, FAM150B and ALK or ALK-F1174L quantified in right picture. Experiments were performed in triplicate and each sample within an experiment was performed in duplicate (error bars indicate SD).
Guan, J., Umapathy, G., Yamazaki, Y., Wolfstetter, G., Mendoza, P., Pfeifer, K., ... & Halenbeck, R. (2015). FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase. elife, 4, e09811.
WB
Figure 5 FAM150A and FAM150B activate ALK.
Whole cell lysates from PC12 cells expressing either vector control, FAM150B, ALK, FAM150B and ALK or ALK-F1174L were analyzed by immunoblot analysis of ALK, pALK-Y1604 (arrowheads), HA (FAM150B) and pERK1/2. Pan-ERK was employed for equal loading.
Guan, J., Umapathy, G., Yamazaki, Y., Wolfstetter, G., Mendoza, P., Pfeifer, K., ... & Halenbeck, R. (2015). FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase. elife, 4, e09811.
Activ
Figure 6 Conditioned medium containing either FAM150A or FAM150B activates endogenous ALK.
Neurite outgrowth in PC12 cells expressing either vector control or anaplastic lymphoma kinase (ALK) were cultured in medium from Human Embryonic Kidney (HEK) 293 cells transfected with either FAM150B.
Guan, J., Umapathy, G., Yamazaki, Y., Wolfstetter, G., Mendoza, P., Pfeifer, K., ... & Halenbeck, R. (2015). FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase. elife, 4, e09811.
WB
Figure 7 Conditioned medium containing either FAM150A or FAM150B activates endogenous ALK.
Whole cell lysates from PC12 cells expressing either vector control or ALK stimulated with medium from HEK293 cells transfected with vector control, FAM150A were analyzed by immunoblot.
Guan, J., Umapathy, G., Yamazaki, Y., Wolfstetter, G., Mendoza, P., Pfeifer, K., ... & Halenbeck, R. (2015). FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase. elife, 4, e09811.
WB
Figure 8 Conditioned medium containing either FAM150A or FAM150B activates endogenous ALK.
IMR32 cells harboring a wild-type ALK receptor were stimulated for 20 min with medium from HEK293 cells transfected with either vector control, FAM150A or FAM150B prior to analysis by immunoblot.
Guan, J., Umapathy, G., Yamazaki, Y., Wolfstetter, G., Mendoza, P., Pfeifer, K., ... & Halenbeck, R. (2015). FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase. elife, 4, e09811.
WB
Figure 9 Conditioned medium containing either FAM150A or FAM150B activates endogenous ALK.
Time course of IMR32 cells harboring a wild type ALK receptor stimulated with FAM150B conditioned medium. Stimulation with ALK activating antibody mAb46 was employed as positive control. Detection of ALK activation was visualized with ALK, pALK-Y1604 (arrowheads), pERK5 and pERK1/2. Pan-ERK was employed for equal loading.
Guan, J., Umapathy, G., Yamazaki, Y., Wolfstetter, G., Mendoza, P., Pfeifer, K., ... & Halenbeck, R. (2015). FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase. elife, 4, e09811.
IP
Figure 10 FAM150A and FAM150B bind to ALK and further activate signaling mediated by the R1275Q ALK neuroblastoma mutation.
FAM150B immunoprecipitates with human ALK. Immunoprecipitation with either anti-FLAG (ALK) or anti-HA (FAM150B) was performed and the resulting immunoprecipitates immunoblotted for the presence of ALK (blue arrowheads) and FAM150B (red arrowheads),*indicates immunoglobulin light and heavy chains.
Guan, J., Umapathy, G., Yamazaki, Y., Wolfstetter, G., Mendoza, P., Pfeifer, K., ... & Halenbeck, R. (2015). FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase. elife, 4, e09811.
IHC
Figure 11 FAM150A and FAM150B bind to ALK and further activate signaling mediated by the R1275Q ALK neuroblastoma mutation.
Human Embryonic Kidney (HEK) 293 cells expressing ALK were incubated with either control or HA-tagged FAM150A or HA-tagged FAM150B conditioned medium prior to analysis by immunohistochemistry. Both HA-tagged FAM150A and HA-tagged FAM150B bind to ALK-expressing cells. Higher magnification panels indicate intracellular vesicles positive for both ALK and HAtagged FAM150A/B.
Guan, J., Umapathy, G., Yamazaki, Y., Wolfstetter, G., Mendoza, P., Pfeifer, K., ... & Halenbeck, R. (2015). FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase. elife, 4, e09811.
WB
Figure 12 FAM150A and FAM150B bind to ALK and further activate signaling mediated by the R1275Q ALK neuroblastoma mutation.
Whole cell lysates from PC12 cells expressing either vector control or ALK-R1275Q were stimulated with medium from HEK293 cells transfected with vector control, FAM150A or FAM150B prior to analysis by immunoblot. Analysis was carried out in the presence or absence of 250 nM crizotinib. Detection of ALK activation was visualized with pALK-Y1604 (arrowheads) and pERK1/2 in whole cell lysates. Pan-ERK was employed for equal loading.
Guan, J., Umapathy, G., Yamazaki, Y., Wolfstetter, G., Mendoza, P., Pfeifer, K., ... & Halenbeck, R. (2015). FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase. elife, 4, e09811.
WB
Figure 13 FAM150A and FAM150B bind to ALK and further activate signaling mediated by the R1275Q ALK neuroblastoma mutation.
CLB-GAR cells harboring the ALK-R1275Q mutant were stimulated for 30 min with medium from HEK293 cells transfected with either vector control, FAM150A or FAM150B prior to analysis by immunoblot. Analysis was carried out in the presence or absence of 250 nM crizotinib. Detection of ALK signaling activation was visualized with pERK1/2. Pan-ERK was employed for equal loading.
Guan, J., Umapathy, G., Yamazaki, Y., Wolfstetter, G., Mendoza, P., Pfeifer, K., ... & Halenbeck, R. (2015). FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase. elife, 4, e09811.
❮
❯
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59545/1417_A10_1_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (HEL, NB-4, RH-30, THP-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000062524-LTK
❮
❯
Specifications
- Host Species
- Human
- Type
- Human antibody
- Specificity
- Human
- Clone
- FAM150B
- Applications
- ELISA, FuncS, FC, WB, Activ, IP, IHC
- Related Disease
- Autoimmune diseases and neurodegenerative diseases
Applications
- Application Notes
- The antibody was validated for ELISA, Functional Assay, Western Blot, Immunoprecipitation, Flow Cytometry, Activation, Surface Plasmon Resonance. For details, refer to Published Data.
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for TAB-0712CL. Click the button above to contact us or submit your feedback about this product.
Excellent For Functional Studies
Clone FAM150B is an incredibly versatile antibody. We have employed it in functional assays and activation studies for Leukocyte Tyrosine Kinase. The binding affinity is strong, and it has helped us elucidate signaling pathways in our autoimmune disease models.
Validated For Many Methods
It is fantastic to have one antibody that works for Flow Cytometry, IP, WB, and ELISA. This human monoclonal antibody against LTK has streamlined our workflow, allowing us to cross-validate our findings using multiple immunological techniques.
Q&As
-
What applications can this antibody be used for?
A: Clone FAM150B is validated for a broad range of applications. These include ELISA, Functional Assays (FuncS), Flow Cytometry (FC), Western Blot (WB), Activation assays (Activ), Immunoprecipitation (IP), and Immunohistochemistry (IHC). It has also been validated using Surface Plasmon Resonance.
-
potential therapeutic areas is this antibody relevant to?
A: While this is for research use, the target LTK (leukocyte tyrosine kinase) is relevant to autoimmune diseases and neurodegenerative diseases. This recombinant human monoclonal antibody allows researchers to investigate the role of LTK in these pathological conditions.
View the frequently asked questions answered by Creative Biolabs Support.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone FAM150B"
- CAT
- Product Name
See other products for "LTK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0711CL | Anti-Human LTK Recombinant Antibody (FAM150A) | ELISA, FuncS, FC, WB, Activ, IP, SPR, IHC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0711CL-S(P) | Anti-Human LTK Recombinant Antibody scFv Fragment (FAM150A) | ELISA, FC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0712CL-S(P) | Anti-Human LTK Recombinant Antibody scFv Fragment (FAM150B) | ELISA, FC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0711CL-F(E) | Anti-Human LTK Recombinant Antibody Fab Fragment (FAM150A) | ELISA, FC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0712CL-F(E) | Anti-Human LTK Recombinant Antibody Fab Fragment (FAM150B) | ELISA, FC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2101 | Hi-Affi™ Recombinant Rabbit Anti-LTK Monoclonal Antibody (DS2101AB) | WB, IHC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0823-LC1 | Mouse Anti-LTK Recombinant Antibody (VS-0823-LC1) | WB, IP, IF, ELISA | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY2025 | Rabbit Anti-LTK Recombinant Antibody (clone R02-8A4) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY177 | AbPlus™ Anti-LTK Magnetic Beads (FAM150A) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC306 | Recombinant Anti-LTK Vesicular Antibody, EV Displayed (VS-0425-YC306) | Activ, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4125 | Anti-LTK Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1025-YC126 | Anti-LTK Antibody Prodrug, Protease Activated (VS-1025-YC126) | ISZ, Cyt, FuncS |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: ELISA, IP, FC, FuncS, Neut, IF, WB
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, WB
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: WB, ELISA, FC, IHC, IP
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, FuncS
Application: ELISA, Block, WB, FC, IP
Application: ELISA, Neut, FuncS
Application: ELISA, Inhib, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
