We need to inhibit NGF in our cell culture system. Is this antibody validated for neutralization?

Yes, this Anti-NGF Antibody is suitable for neutralization studies. It has been validated for its ability to bind to Nerve Growth Factor (NGF) and inhibit its biological activity in vitro. This makes it an excellent tool for experiments where you need to block NGF signaling to assess its effects on neuronal survival, differentiation, or other physiological processes.

Does this antibody recognize mature NGF?

Yes, this antibody is designed to recognize the active, mature form of Nerve Growth Factor. It binds to the secreted neurotrophin, allowing for both detection in supernatants and functional inhibition. We recommend it for research involving the characterization of NGF levels and activity in your biological models.

Recombinant Rabbit Anti-NGF Antibody (CBL1010)

CAT#: NEUT-1774CQ

This antibody is a Rabbit monoclonal antibody that binds specifically to NGFB, and it can neutralize the bioactivity of NGFB.

Gene Expression

RNA Expression Figure 1 RNA cell line category: Cell line enhanced.

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000134259-NGF

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Specifications

Immunogen
Recombinant Human NGF/NGFB Protein

Host Species
Rabbit

Type
IgG

Specificity
Human NGF/NGFB

Species Reactivity
Human

Clone
CBL1010

Applications
Used for immunoassay techniques such as: Neutralization

Conjugate
Unconjugated

Product Property

Purity
>95% as determined by analysis by SDS-PAGE

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze-thaw cycles.

Target

Alternative Names
NGF; nerve growth factor; NGFB; HSAN5; Beta-NGF

Gene ID
4803

UniProt ID
P01138

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Breast cancer biomarkers at key points during disease progression

Effective NGF Neutralization

We purchased this antibody specifically for its neutralizing capabilities. We tested it in a PC12 cell differentiation assay, and it successfully inhibited Nerve Growth Factor-induced neurite outgrowth in a dose-dependent manner. The potency was high, requiring only small amounts to achieve significant inhibition. This has allowed us to dissect the specific contributions of NGF signaling in our neurobiology models.

Robust Functional Antibody

This Anti-NGF antibody is a robust tool for functional studies. Aside from neutralization, we also checked its performance in Western Blot, where it recognized the mature NGF protein clearly. The versatility of being able to use it for both detection and functional inhibition makes it valuable. The product arrived on dry ice and was active immediately upon thawing.

Q&As

We need to inhibit NGF in our cell culture system. Is this antibody validated for neutralization?

A: Yes, this Anti-NGF Antibody is suitable for neutralization studies. It has been validated for its ability to bind to Nerve Growth Factor (NGF) and inhibit its biological activity in vitro. This makes it an excellent tool for experiments where you need to block NGF signaling to assess its effects on neuronal survival, differentiation, or other physiological processes.
Does this antibody recognize mature NGF?

A: Yes, this antibody is designed to recognize the active, mature form of Nerve Growth Factor. It binds to the secreted neurotrophin, allowing for both detection in supernatants and functional inhibition. We recommend it for research involving the characterization of NGF levels and activity in your biological models.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - NEUT-1774CQ.pdf

MSDS

MSDS - NEUT-1774CQ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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