Rat Anti-NGF Recombinant Antibody (clone αD11) (CAT#: PABX-146-S (P))

Figure 1 IP and WB of transgenic and wild-type mice.

IP and WB of cortex extracts from male(M) and female(F) TgProNGF#72 and wild-type (WT) mice. IP on extracts from cortex(CTX) with anti-NGF aD11 antibody, followed by WB with anti-NGF or anti-proNGF antibody, as described in Tiveron et al.(2013). Are presentative WB probed with anti-proNGF(PAb Alomone),(top)or anti-NGFM20 (Santa Cruz)(bottom) is shown. TgproNGF#72 and wild type mice,male and female,were analyzed.

Malerba, F., Paoletti, F., & Cattaneo, A. (2016). NGF and proNGF reciprocal interference in immunoassays: open questions, criticalities, and ways forward. Frontiers in molecular neuroscience, 9, 63.

Figure 2 EmaxNGF Promega:NGFS tandard curves.

NGF standard curves carried out by usingr at mAb Promega(triangles) and mAb aD11(Cattaneoetal.,1988) (squares) as primary antibody.

Malerba, F., Paoletti, F., & Cattaneo, A. (2016). NGF and proNGF reciprocal interference in immunoassays: open questions, criticalities, and ways forward. Frontiers in molecular neuroscience, 9, 63.

Figure 3 SPR analysis-calibration curve sobtained with proNGF and NG Fover the panel of different antibodies.

Different pro NGF concentrations tested on anti-NGFmAb aD11. ConcentrationsinnM(fromtop): 500,333,222,148,98, 65,33,16,8,4,2,1,0.5.

Malerba, F., Paoletti, F., & Cattaneo, A. (2016). NGF and proNGF reciprocal interference in immunoassays: open questions, criticalities, and ways forward. Frontiers in molecular neuroscience, 9, 63.