Mouse Anti-NPC2 Recombinant Antibody (clone 3-6B) (CAT#: HPAB-0225-LSX)
Recombinant monoclonal antibody to NPC2. Clone 3-6B is a mouse IgG2a that can be potentially used in the non-alcoholic steatohepatitis.
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WB
Figure 1 Western blot analysis using the mAbs (14-8D, 5-5B, 5-4B, 4-12C, 3-6B, 2-7D, 1-5B and 1-2G) against GST-NPC2 and His-NPC2.
Liao, Y. J., Lin, M. W., Yen, C. H., Lin, Y. T., Wang, C. K., Huang, S. F.,... & Chen, Y. M. A. (2013). Characterization of Niemann-Pick Type C2 protein expression in multiple cancers using a novel NPC2 monoclonal antibody. PLoS One, 8(10).
WB
Figure 2 Western blot analysis using the mAbs against mouse epididymis.
Liao, Y. J., Lin, M. W., Yen, C. H., Lin, Y. T., Wang, C. K., Huang, S. F.,... & Chen, Y. M. A. (2013). Characterization of Niemann-Pick Type C2 protein expression in multiple cancers using a novel NPC2 monoclonal antibody. PLoS One, 8(10).
WB
Figure 3 Epitope region mapping of the monoclonal NPC2 antibodies.
Different length of pNPC2-HA including full-length pNPC2-HA, N-terminal half (1-80 a.a.), C-terminal half (81-151 a.a.) and 41-105 a.a. were transfected into 293T cells and 24 hrs later were harvested for Western blot analysis. Lane 1, 1-2G; Land 2, 1-5B; Lane 3, 2-7B; Lane 4, 3-6B; Lane 5, mAb-HA; Lane 6, 4-12C; Lane 7, 5-4B, Lane 8, 5-5B; Lane 9, 14-8D.
Liao, Y. J., Lin, M. W., Yen, C. H., Lin, Y. T., Wang, C. K., Huang, S. F.,... & Chen, Y. M. A. (2013). Characterization of Niemann-Pick Type C2 protein expression in multiple cancers using a novel NPC2 monoclonal antibody. PLoS One, 8(10).
ELISA
Figure 4 An ELISA plate coated with four peptides covering the 1-40 a.a. region of NPC2 protein was used for epitope mapping.
Representative results from the mAbs (14-8D, 5-4B, 3-6B, 2-7D, 1-5B and 1-2G) are shown.
Liao, Y. J., Lin, M. W., Yen, C. H., Lin, Y. T., Wang, C. K., Huang, S. F.,... & Chen, Y. M. A. (2013). Characterization of Niemann-Pick Type C2 protein expression in multiple cancers using a novel NPC2 monoclonal antibody. PLoS One, 8(10).
WB
Figure 5 The knockdown effect of shNPC2 on SK-Hep1 cells was detected using anti-NPC2 mAbs (3-6B, 14-8D, 5-4B and 2-7B).
Liao, Y. J., Lin, M. W., Yen, C. H., Lin, Y. T., Wang, C. K., Huang, S. F.,... & Chen, Y. M. A. (2013). Characterization of Niemann-Pick Type C2 protein expression in multiple cancers using a novel NPC2 monoclonal antibody. PLoS One, 8(10).
Specifications
- Immunogen
- GST-NPC2
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human NPC2
- Species Reactivity
- Human
- Clone
- 3-6B
- Applications
- ELISA, WB
- Related Disease
- Non-alcoholic steatohepatitis
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1717MZ | Recombinant Mouse Anti-Human NPC2 Antibody | ELISA, WB | Mouse antibody |
| VS3-FY2048 | Rabbit Anti-NPC2 Recombinant Antibody (clone R07-0C8) | WB, IHC-Fr, IHC-P, IF | Rabbit IgG |
| VS13-YC834 | Rabbit Anti-NPC2 Recombinant Antibody (VS13-YC834) | WB, IHC-P | Rabbit IgG |
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