Recombinant Mouse Anti-P. falciparum AMA1 Antibody (1F9) (CAT#: PABW-097)

Figure 1 ELISA analysis of MAb 1F9, 5G8, 2C5 reactivity with recombinant refolded AMA1 derived from P. falciparum strains 3D7, D10, HB3, and W2mef. AMA1 preparations were adsorbed onto the wells, and antibodies were then allowed to react at the indicated concentrations.

Absorbances at 450 nm and MAb concentrations are indicated on the ordinate and abscissa, respectively.

Coley, A. M. . (2006). The most polymorphic residue on plasmodium falciparum apical membrane antigen 1 determines binding of an invasion-inhibitory antibody. Infection & Immunity, 74(5), 2628.

Figure 2 Western analysis of AMA1 expressed by parasite strains 3D7, D10, HB3, and W2mef. Schizont-stage parasites were separated by SDS-PAGE and blotted onto polyvinylidene difluoride, and blots were visualized with Mabs 1F9, 2C5, and 5G8 and rabbit anti-AMA1.

Arrows indicate the locations of the unprocessed 83-kDa and processed 66-kDa bands.

Coley, A. M. . (2006). The most polymorphic residue on plasmodium falciparum apical membrane antigen 1 determines binding of an invasion-inhibitory antibody. Infection & Immunity, 74(5), 2628.

Figure 3 Growth inhibition assays of 3D7, D10, HB3, and W2mef parasites.

Each antibody, i.e., 1F9 (A), 2C5 (B), and rabbit anti-AMA1 (C), was assayed for its growth inhibition qualities. Data points indicate the means of three identical wells, and error bars indicate one standard deviation from the mean.

Coley, A. M. . (2006). The most polymorphic residue on plasmodium falciparum apical membrane antigen 1 determines binding of an invasion-inhibitory antibody. Infection & Immunity, 74(5), 2628.