Recombinant Mouse Anti-P. falciparum AMA1 Antibody (1F9) (CAT#: PABW-097)
Recombinant Mouse Antibody (1F9) is capable of binding to P. falciparum AMA1, expressed in Chinese Hamster Ovary cells (CHO). This antibody could inhibit merozoite invasion by several mechanisms.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Specifications
- Immunogen
- P. falciparum apical membrane antigen 1
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native P. falciparum AMA1
- Species Reactivity
- P. falciparum
- Clone
- 1F9
- Applications
- ELISA, BL
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The AMA1 antibody has been reported in applications of ELISA, WB, Inhibition.
WB: The membrane was blocked with 10% skim milk in phosphatebuffered saline (PBS) and developed with 2 ug ml 1 of either MAb 1F9, 2C5, or 5G8 or a pool of rabbit anti-3D7, -D10, -HB3, and -W2mef AMA1 antisera in PBS, followed by 1g ml 1 peroxidase-conjugated rabbit anti-mouse or goat anti-rabbit immunoglobulin G (Chemicon) accordingly.
ELISA: For assays involving binding of recombinant refolded AMA1 to MAbs 1F9, 2C5, and 5G8, 100 l AMA1 (1 ug/ml) in coating buffer (0.1 M sodium carbonate, pH 9.5) was applied to Maxisorp (Nunc) enzyme-linked immunosorbent assay (ELISA) plate wells overnight at 4°C.
Target
- Alternative Names
- AMA1; apical membrane antigen 1
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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CAT | Product Name | Application | Type |
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PABL-293 | Recombinant Rat Anti-P. Knowlesi AMA-1 Antibody (R31C2 ) | WB, BL, FuncS | IgG |
PABZ-098 | Recombinant Mouse Anti-P. vivax AMA-1 Antibody (F8.12.19) | IF | IgG |
PABZ-148 | Recombinant Mouse Anti-P. vivax AMA1 Antibody (F8.12.19) | WB | IgG |
PABL-397 | Human Anti-AMA1 Recombinant Antibody (PABL-397) | WB, ELISA, FuncS | Human IgG |
MOB-025CQ | Rabbit Anti-P. falciparum AMA1 Antibody | ELISA, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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