Recombinant Mouse Anti-P. vivax AMA-1 Antibody (F8.12.19) (CAT#: PABZ-098)
Recombinant Mouse Antibody (F8. 12. 19) is capable of binding to P. vivax AMA-1, expressed in Chinese Hamster Ovary cells (CHO). This antibody recognises a discontinuous epitope located on domain III of the ectoplasmic region, the major component being a loop containing a cystine knot.
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Figure 2 RON2-AMA1 interaction is conserved in P. falciparum.
(A) Pf3D7-AMA1 displays a type I TM topology at the plasma membrane of BHK-21 cells. PfAMA1 transfected BHK-21 cells were subjected to IFA. Mouse MAb F8.12.19 recognizes extracellular PfAMA1 domain III that is accessible with or without permeabilization, whereas anti-Myc antibody labels the intracellular C-terminal Myc tag in permeabilized cells only. (B) PfAMA1 transfected BHK-21 cells were incubated with 20 µg/ml of GST or PfRON2-5. Recombinant proteins were detected using anti-GST antibody.
Lamarque, M., Besteiro, S., Papoin, J., Roques, M., Vulliez-Le Normand, B., Morlon-Guyot, J., ... & Kocken, C. H. (2011). The RON2-AMA1 interaction is a critical step in moving junction-dependent invasion by apicomplexan parasites. PLoS pathogens, 7(2), e1001276.
Specifications
- Immunogen
- P. vivax apical membrane antigen 1, AMA1
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native P. vivax AMA-1
- Species Reactivity
- P. vivax
- Clone
- F8.12.19
- Applications
- IF 1 µg/ml
WB 2 μg/mL
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody F8.12.19 has been reported in applications of IF, WB. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
IF: PfAMA1 transfected BHK-21 cells were subjected to IFA. The reported concentration of the antibody F8.12.19 for IF was 1 µg/ml.
WB: The reported concentration of the antibody F8.12.19 for WB was 2 µg/ml.
Target
- Alternative Names
- AMA-1; Pf83; RMA-1; RMA1
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-293 | Recombinant Rat Anti-P. Knowlesi AMA-1 Antibody (R31C2 ) | WB, BL, FuncS | IgG |
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| PABL-397 | Human Anti-AMA1 Recombinant Antibody (PABL-397) | WB, ELISA, FuncS | Human IgG |
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| MOB-025CQ | Rabbit Anti-P. falciparum AMA1 Antibody | ELISA, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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