Recombinant Anti-human PABPN1 Intrabody [Tat]
CAT#: IAB-B060(T)
Recombinant anti-PABPN1 intrabody in fusion with HIV Tat works within cells to bind human PABPN1, expressed in E. coli cells.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1899_A4_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_8_4.jpg
Normal Tissue
Figure 5 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_3_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100836-PABPN1
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Specifications
- Immunogen
- human poly(A) binding protein, nuclear 1
- Host Species
- llama
- Type
- vhh-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Products can be used for: Fluorescence-activated cell sorting; Enzyme-linked Immunosorbent Assay; Immunofluorescence; Functional studies
Product Property
- Purity
- >95% by HPLC
- Storage
- ≥1 year at -20°C. If the reconstituted antibody cannot be used within two weeks, it should be aliquoted into smaller vials and stored at -20°C
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "PABPN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2170z | Mouse Anti-PABPN1 Recombinant Antibody (clone 18H9) | WB, ELISA, IHC | Mouse IgG1 |
| MRO-1160-CN | Recombinant Rabbit Anti-PABPN1 Monoclonal Antibody (JM11-28) | WB, IF, IHC, FC, IP | Rabbit IgG |
| ZG-0494U | Rabbit Anti-PABPN1 Recombinant Antibody (clone 6C3) | ELISA, WB, IHC, IF, FC | Rabbit IgG |
| VS3-FY1812 | Rabbit Anti-PABPN1 Recombinant Antibody (clone R02-8B1) | WB, IHC-Fr, IHC-P, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-021 | Recombinant Anti-human PABPN1 VHH Single Domain Antibody | WB, ELISA, IHC, FC, FuncS | Llama VHH |
| HPAB-1228WJ | Recombinant Llama Anti-PABPN1 Single Domain Antibody (HPAB-1228WJ) | ELISA | Llama VHH |
| HPAB-1229WJ | Recombinant Llama Anti-PABPN1 Single Domain Antibody (HPAB-1229WJ) | ELISA | Llama VHH |
| HPAB-1230WJ | Recombinant Llama Anti-PABPN1 Single Domain Antibody (HPAB-1230WJ) | ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B060(A) | Recombinant Anti-human PABPN1 Intrabody [(D-Arg)9] | FACS, ICC, FuncS | vhh-(D-Arg)9 |
| IAB-B060(G) | Recombinant Anti-human PABPN1 Intrabody [+36 GFP] | ELISA, ICC, FuncS | vhh-(+36GFP) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2572 | Hi-Affi™ Recombinant Rabbit Anti-PABPN1 Monoclonal Antibody (DS2572AB) | FC, ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1554 | Anti-PABPN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5146 | Anti-Human PABPN1 Immunohistochemistry Kit | IHC |
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