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    Recombinant Anti-human PABPN1 Intrabody [Tat] (CAT#: IAB-B060(T))

    Recombinant anti-PABPN1 intrabody in fusion with HIV Tat works within cells to bind human PABPN1, expressed in E. coli cells.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

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    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1899_A4_2_selected.jpg
    Normal Tissue
    Figure 2 Cerebral cortex
    Figure 2 Cerebral cortex
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_B_8_5.jpg
    Figure 3 Colon
    Figure 3 Colon
    (Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_7_3.jpg
    Figure 4 Liver
    Figure 4 Liver
    (Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_8_4.jpg
    Figure 5 Pancreas
    Figure 5 Pancreas
    (Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_3_3.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_9_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_6_6.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_9_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Low cell line specificity
    Figure 9 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100836-PABPN1

    Specifications

    • Immunogen
    • human poly(A) binding protein, nuclear 1
    • Host Species
    • llama
    • Type
    • vhh-Tat
    • Cell Penetrating Peptide
    • TAT
      TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
    • Species Reactivity
    • human
    • Applications
    • Products can be used for: Fluorescence-activated cell sorting; Enzyme-linked Immunosorbent Assay; Immunofluorescence; Functional studies

    Product Property

    • Purity
    • >95% by HPLC
    • Storage
    • ≥1 year at -20°C. If the reconstituted antibody cannot be used within two weeks, it should be aliquoted into smaller vials and stored at -20°C

    Target

    • Alternative Names
    • PABPN1; poly(A) binding protein, nuclear 1; OPMD; PAB2; PABII; PABP2; PABP-2; polyadenylate-binding protein 2; poly(A) binding protein 2; poly(A) binding protein II

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

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    See other products for "PABPN1"

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    MOB-2170z Mouse Anti-PABPN1 Recombinant Antibody (clone 18H9) WB, ELISA, IHC Mouse IgG1
    MRO-1160-CN Recombinant Rabbit Anti-PABPN1 Monoclonal Antibody (JM11-28) WB, IF, IHC, FC, IP Rabbit IgG
    ZG-0494U Rabbit Anti-PABPN1 Recombinant Antibody (clone 6C3) ELISA, WB, IHC, IF, FC Rabbit IgG
    VS3-FY1812 Rabbit Anti-PABPN1 Recombinant Antibody (clone R02-8B1) WB, IHC-Fr, IHC-P, IF, IP Rabbit IgG

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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