Rabbit Anti-PABPN1 Recombinant Antibody (clone R02-8B1)
CAT#: VS3-FY1812
This R02-8B1 recombinant monoclonal antibody with high affinity for human PABPN1. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. To validate the QC result of the antibody, we recommend a dilution for the antibody by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). Applications Tested: The PABPN1 antibody has been tested by western blot analysis of PABPN1 in Jurkat, Hela, COS7, Raji lysates and immunohistochemistry analysis of paraffin-embedded human breast cancer.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1899_A4_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_8_4.jpg
Normal Tissue
Figure 5 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_3_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/637/1472_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100836-PABPN1
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WB
Figure 1 Anti-PABPN1 Antibody (VS3-FY1812) in WB.
The PABPN1 expression in Jurkat, Hela, COS7, Raji lysates were detected using the anti-PABPN1 by western blot assay.
IHC
Figure 2 Anti-PABPN1 Antibody (VS3-FY1812) in IHC.
Immunohistochemistry staining of paraffin-embedded human breast cancer using the anti-PABPN1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human PABPN1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse PABPN1
- Species Reactivity
- Human, Mouse
- Clone
- R02-8B1
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 33 kDa, while the observed molecular weight stands at 50 kDa.
- Related Disease
- Human breast cancer, Autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Poly(A) Binding Protein Nuclear 1; PABP-2; PABP2; PABII; PAB2; Polyadenylate-Binding Nuclear Protein 1; Poly(A)-Binding Protein, Nuclear 1; Poly(A) Binding Protein, Nuclear 1
- Gene ID
- 8106
- UniProt ID
- Q86U42
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Arginine dimethylation is asymmetric and involves PRMT1 and PRMT3.
It does not influence the RNA binding properties ().
(Q86U42-PABP2_HUMAN)
Ubiquitination at Lys123 (NX_Q86U42 [NX_Q86U42-1])
Modification sites at PhosphoSitePlus (Q86U42)
Glycosylation from GlyGen (Q86U42) 7 sites, 1 O-linked glycan (7 sites)
- Protein Refseq
- NP_001347480.1; NP_001347481.1; NP_004634.1
- Function
- Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (By similarity).
Increases the affinity of poly(A) polymerase for RNA (By similarity).
Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PABPN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2170z | Mouse Anti-PABPN1 Recombinant Antibody (clone 18H9) | WB, ELISA, IHC | Mouse IgG1 |
| MRO-1160-CN | Recombinant Rabbit Anti-PABPN1 Monoclonal Antibody (JM11-28) | WB, IF, IHC, FC, IP | Rabbit IgG |
| ZG-0494U | Rabbit Anti-PABPN1 Recombinant Antibody (clone 6C3) | ELISA, WB, IHC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-021 | Recombinant Anti-human PABPN1 VHH Single Domain Antibody | WB, ELISA, IHC, FC, FuncS | Llama VHH |
| HPAB-1228WJ | Recombinant Llama Anti-PABPN1 Single Domain Antibody (HPAB-1228WJ) | ELISA | Llama VHH |
| HPAB-1229WJ | Recombinant Llama Anti-PABPN1 Single Domain Antibody (HPAB-1229WJ) | ELISA | Llama VHH |
| HPAB-1230WJ | Recombinant Llama Anti-PABPN1 Single Domain Antibody (HPAB-1230WJ) | ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B060(A) | Recombinant Anti-human PABPN1 Intrabody [(D-Arg)9] | FACS, ICC, FuncS | vhh-(D-Arg)9 |
| IAB-B060(G) | Recombinant Anti-human PABPN1 Intrabody [+36 GFP] | ELISA, ICC, FuncS | vhh-(+36GFP) |
| IAB-B060(T) | Recombinant Anti-human PABPN1 Intrabody [Tat] | FACS, ELISA, IF, FuncS | vhh-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2572 | Hi-Affi™ Recombinant Rabbit Anti-PABPN1 Monoclonal Antibody (DS2572AB) | FC, ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1554 | Anti-PABPN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5146 | Anti-Human PABPN1 Immunohistochemistry Kit | IHC |
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