Rabbit Anti-PABPN1 Recombinant Antibody (clone R02-8B1) (CAT#: VS3-FY1812)
This R02-8B1 recombinant monoclonal antibody with high affinity for human PABPN1. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. To validate the QC result of the antibody, we recommend a dilution for the antibody by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). Applications Tested: The PABPN1 antibody has been tested by western blot analysis of PABPN1 in Jurkat, Hela, COS7, Raji lysates and immunohistochemistry analysis of paraffin-embedded human breast cancer.
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WB
Figure 1 Anti-PABPN1 Antibody (VS3-FY1812) in WB.
The PABPN1 expression in Jurkat, Hela, COS7, Raji lysates were detected using the anti-PABPN1 by western blot assay.
IHC
Figure 2 Anti-PABPN1 Antibody (VS3-FY1812) in IHC.
Immunohistochemistry staining of paraffin-embedded human breast cancer using the anti-PABPN1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human PABPN1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse PABPN1
- Species Reactivity
- Human, Mouse
- Clone
- R02-8B1
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 33 kDa, while the observed molecular weight stands at 50 kDa.
- Related Disease
- Human breast cancer, Autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Poly(A) Binding Protein Nuclear 1; PABP-2; PABP2; PABII; PAB2; Polyadenylate-Binding Nuclear Protein 1; Poly(A)-Binding Protein, Nuclear 1; Poly(A) Binding Protein, Nuclear 1
- Gene ID
- 8106
- UniProt ID
- Q86U42
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Arginine dimethylation is asymmetric and involves PRMT1 and PRMT3.
It does not influence the RNA binding properties ().
(Q86U42-PABP2_HUMAN)
Ubiquitination at Lys123 (NX_Q86U42 [NX_Q86U42-1])
Modification sites at PhosphoSitePlus (Q86U42)
Glycosylation from GlyGen (Q86U42) 7 sites, 1 O-linked glycan (7 sites)
- Protein Refseq
- NP_001347480.1; NP_001347481.1; NP_004634.1
- Function
- Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (By similarity).
Increases the affinity of poly(A) polymerase for RNA (By similarity).
Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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Intrabody Products
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2170z | Mouse Anti-PABPN1 Recombinant Antibody (clone 18H9) | WB, ELISA, IHC | Mouse IgG1 |
| MRO-1160-CN | Recombinant Rabbit Anti-PABPN1 Monoclonal Antibody (JM11-28) | WB, IF, IHC, FC, IP | Rabbit IgG |
| ZG-0494U | Rabbit Anti-PABPN1 Recombinant Antibody (clone 6C3) | ELISA, WB, IHC, IF, FC | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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