Anti-PMEL immunotoxin (Fab)-PE (AGTO-G051E)
This immunotoxin was achieved by conjugating/fusing the Anti-PMEL Fab to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to cancer cells. Once bind, the toxin enters cells by receptor-mediated endocytosis. Then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treating Human melanoma Xenografts.
Specifications
- Host
- Mouse
- Species Reactivity
- Human
- Molecule Class
- Fab-PE
- Toxin Description
- Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- Human Melanoma Xenografts
Target
- Alternative Names
- PMEL; premelanosome protein; P1; SI; SIL; ME20; P100; SILV
- Entrez Gene ID
- 6490
- UniProt ID
- P40967
For lab research use only, not for diagnostic, therapeutic or any in vivo human use.
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