Recombinant Mouse Anti-Prostaglandin E2 Antibody (PGE2) (CAT#: PABX-160)

Anti-Prostaglandin E2 antibody is a Mouse antibody of IgG class that binds to an Prostaglandin E2.


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Figure 1 PGE2 results in deacetylation of chromatin associated with the 15-PGDH gene promoter.

Figure 1 PGE2 results in deacetylation of chromatin associated with the 15-PGDH gene promoter.

(A) Chromatin immunoprecipitation (IP) of acetylated histone H3 (AcH3) is compared with IP of IgG (negative control) in cells treated with DMSO or PGE2 (100 nM) for 24 h. Data represent average fold enrichment. *P < 0.05 compared with DMSO, Student's t test. n = 3. (B) Representative immunoblot and quantitation of 15-PGDH levels in hCSCs treated with DMSO or HDACi SAHA (2.5 μM), TSA (1 μM), or HDAC-42 (1 μM) for 24 h. Acetylated histone H3(AcH3), positive control; β-actin, loading control. *P < 0.05 compared with DMSO, Student's t test. n = 3. (C) Dose-(24 h) and time-dependent [SAHA (2.5 μM), TSA (1 μM), or HDAC-42 (1 μM)] increases in 15-PGDH after treatment with HDACi. *P < 0.05 compared with DMSO (C) or 0 time point (D), ANOVA, Dunn's post hoc testing. n = 3. (E) Data represent fragments per kilobase of exon per million fragments mapped (FPKM) of class I, IIA, IIB, and IV HDACs expressed in hCSCs treated
with DMSO or PGE2 (100 nM) for 1 or 24 h mined from the RNA-seq dataset.
GAPDH and RPLP0 are shown as controls (1/100th FPKM values). *P < 0.05 compared with corresponding DMSO control.

Kishore, A. H., Liang, H., Kanchwala, M., Xing, C., Ganesh, T., Akgul, Y., ... & Word, R. A. (2017). Prostaglandin dehydrogenase is a target for successful induction of cervical ripening. Proceedings of the National Academy of Sciences, 114(31), E6427-E6436.

Figure 2 Western Blot Analysis of HDAC4 protein levels.

Figure 2 Western Blot Analysis of HDAC4 protein levels.

HDAC4 protein levels in hCSCs pretreated with DMSO or PF-04418948 (2 μM) followed by DMSO or PGE2. β-actin, loading control.

Kishore, A. H., Liang, H., Kanchwala, M., Xing, C., Ganesh, T., Akgul, Y., ... & Word, R. A. (2017). Prostaglandin dehydrogenase is a target for successful induction of cervical ripening. Proceedings of the National Academy of Sciences, 114(31), E6427-E6436.

Figure 3 Masson’s trichrome stain of midcervical transverse sections of mice treated with (a) vehicle, (b) PGE2, (c) SW033291, or (d) PGE2 + SW033291.

Figure 3 Masson’s trichrome stain of midcervical transverse sections of mice treated with (a) vehicle, (b) PGE2, (c) SW033291, or (d) PGE2 + SW033291.

Animals were treated bidaily on d 15 and d 16 with tissue collection on late d 16 before delivery. (Bar, 200 μm.) epi, epithelium; str, stroma.

Kishore, A. H., Liang, H., Kanchwala, M., Xing, C., Ganesh, T., Akgul, Y., ... & Word, R. A. (2017). Prostaglandin dehydrogenase is a target for successful induction of cervical ripening. Proceedings of the National Academy of Sciences, 114(31), E6427-E6436.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native Human Prostaglandin E2
  • Species Reactivity
  • Human
  • Clone
  • PGE2
  • Applications
  • Neut (assay dependent)
    FuncS (assay dependent)

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • Assay-depended

Target

  • Alternative Names
  • PGE2; Prostaglandin E2; PGE2; 2B5

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone PGE2"

See other products for "PGE2"

Chimeric Antibody

CAT Product Name Application Type
TAB-1023CL Anti-Human PGE2 Recombinant Antibody (2B5-7.0) ELISA Chimeric antibody (mouse/human)
TAB-1023CL-S(P) Anti-Human PGE2 Recombinant Antibody scFv Fragment (2B5-7.0) ELISA Chimeric antibody (mouse/human)
TAB-1023CL-F(E) Anti-Human PGE2 Recombinant Antibody Fab Fragment (2B5-7.0) ELISA Chimeric antibody (mouse/human)

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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