Recombinant Mouse Anti-Prostaglandin E2 Antibody (PGE2)
CAT#: PABX-160
Anti-Prostaglandin E2 antibody is a Mouse antibody of IgG class that binds to an Prostaglandin E2.
Published Data
Figure 1 PGE2 results in deacetylation of chromatin associated with the 15-PGDH gene promoter.
(A) Chromatin immunoprecipitation (IP) of acetylated histone H3 (AcH3) is compared with IP of IgG (negative control) in cells treated with DMSO or PGE2 (100 nM) for 24 h. Data represent average fold enrichment. *P < 0.05 compared with DMSO, Student's t test. n = 3. (B) Representative immunoblot and quantitation of 15-PGDH levels in hCSCs treated with DMSO or HDACi SAHA (2.5 μM), TSA (1 μM), or HDAC-42 (1 μM) for 24 h. Acetylated histone H3(AcH3), positive control; β-actin, loading control. *P < 0.05 compared with DMSO, Student's t test. n = 3. (C) Dose-(24 h) and time-dependent [SAHA (2.5 μM), TSA (1 μM), or HDAC-42 (1 μM)] increases in 15-PGDH after treatment with HDACi. *P < 0.05 compared with DMSO (C) or 0 time point (D), ANOVA, Dunn's post hoc testing. n = 3. (E) Data represent fragments per kilobase of exon per million fragments mapped (FPKM) of class I, IIA, IIB, and IV HDACs expressed in hCSCs treated
with DMSO or PGE2 (100 nM) for 1 or 24 h mined from the RNA-seq dataset.
GAPDH and RPLP0 are shown as controls (1/100th FPKM values). *P < 0.05 compared with corresponding DMSO control.
Kishore, A. H., Liang, H., Kanchwala, M., Xing, C., Ganesh, T., Akgul, Y., ... & Word, R. A. (2017). Prostaglandin dehydrogenase is a target for successful induction of cervical ripening. Proceedings of the National Academy of Sciences, 114(31), E6427-E6436.
Figure 2 Western Blot Analysis of HDAC4 protein levels.
HDAC4 protein levels in hCSCs pretreated with DMSO or PF-04418948 (2 μM) followed by DMSO or PGE2. β-actin, loading control.
Kishore, A. H., Liang, H., Kanchwala, M., Xing, C., Ganesh, T., Akgul, Y., ... & Word, R. A. (2017). Prostaglandin dehydrogenase is a target for successful induction of cervical ripening. Proceedings of the National Academy of Sciences, 114(31), E6427-E6436.
Figure 3 Masson’s trichrome stain of midcervical transverse sections of mice treated with (a) vehicle, (b) PGE2, (c) SW033291, or (d) PGE2 + SW033291.
Animals were treated bidaily on d 15 and d 16 with tissue collection on late d 16 before delivery. (Bar, 200 μm.) epi, epithelium; str, stroma.
Kishore, A. H., Liang, H., Kanchwala, M., Xing, C., Ganesh, T., Akgul, Y., ... & Word, R. A. (2017). Prostaglandin dehydrogenase is a target for successful induction of cervical ripening. Proceedings of the National Academy of Sciences, 114(31), E6427-E6436.
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Antibody Isotype
- IgG
- Specificity
- Tested positive against native Human Prostaglandin E2
- Species Reactivity
- Human
- Clone
- PGE2
- Applications
- Neut (assay dependent)
FuncS (assay dependent)
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
- Alternative Names
- PGE2; Prostaglandin E2; PGE2; 2B5
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Effective For Small Molecule Targets
Targeting small lipids like Prostaglandin E2 can be tricky, but this antibody worked very well. We used it in a neutralization assay, and it effectively bound to PGE2, modulating its effects in our system. The antibody appears to be of high quality and specificity for the dinoprostone molecule.
Useful For Functional Studies
We incorporated this Recombinant Mouse Antibody into our functional studies regarding inflammation resolution. It demonstrated assay-dependent neutralization capabilities that were crucial for verifying the role of PGE2 in our specific pathway. The purity and stability of the reagent were satisfactory for our rigorous experimental needs.
Q&As
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What type of molecule does this antibody detect?
A: This antibody detects Prostaglandin E2 (PGE2), which is a small lipid molecule involved in inflammation and other physiological processes. Unlike antibodies against proteins, this antibody is designed to bind to the chemical structure of PGE2, making it useful for neutralizing or detecting this specific metabolite.
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Is this antibody capable of neutralization?
A: Yes, this Recombinant Mouse Anti-Prostaglandin E2 Antibody is validated for Neutralization (Neut) and Functional Studies (FuncS). The extent of neutralization is assay-dependent, so we recommend optimizing the conditions to suit your specific experimental system and biological questions.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Diseases
Rheumatoid Arthritis
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-765 | Recombinant Anti-PGE2 Antibody | ELISA, WB, FC, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-765-F(E) | Recombinant Anti-PGE2 Antibody Fab Fragment | IF, FACS, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-765-S(P) | Recombinant Anti-PGE2 Antibody scFv Fragment | IF, FC, Biosensors, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-765 | Recombinant Human Anti-PGE2 Antibody | WB, ELISA, IHC, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-765-F(E) | Recombinant Human Anti-PGE2 Antibody Fab Fragment | WB, RIA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-765-S(P) | Recombinant Human Anti-PGE2 Antibody scFv Fragment | WB, IF, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1022CL | Mouse Anti-PGE2 Recombinant Antibody (TAB-1022CL) | FuncS, ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1023CL | Human Anti-PGE2 Recombinant Antibody (TAB-1023CL) | ELISA | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1022CL-S(P) | Mouse Anti-PGE2 Recombinant Antibody scFv Fragment (TAB-1022CL-S(P)) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1023CL-S(P) | Human Anti-PGE2 Recombinant Antibody scFv Fragment (TAB-1023CL-S(P)) | ELISA | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1022CL-F(E) | Mouse Anti-PGE2 Recombinant Antibody Fab Fragment (TAB-1022CL-F(E)) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1023CL-F(E) | Human Anti-PGE2 Recombinant Antibody Fab Fragment (TAB-1023CL-F(E)) | ELISA | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-160-S (P) | Recombinant Mouse Anti-Prostaglandin E2 Antibody scFv Fragment (PGE2) | Neut, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-160-F (E) | Recombinant Mouse Anti-Prostaglandin E2 Antibody Fab Fragment (PGE2) | Neut, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-080CQ | Human Anti-PGE2 Recombinant Antibody (MOB-080CQ) | ELISA, Neut | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0731-CN | Human Anti-PGE2 Recombinant Antibody (HPAB-0731-CN) | ELISA, Neut | Chimeric (mouse/human) IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0732-CN | Human Anti-PGE2 Recombinant Antibody (HPAB-0732-CN) | ELISA, Neut | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0731-CN-S(P) | Mouse Anti-PGE2 Recombinant Antibody; scFv Fragment (HPAB-0731-CN-S(P)) | ELISA, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0732-CN-S(P) | Human Anti-PGE2 Recombinant Antibody; scFv Fragment (HPAB-0732-CN-S(P)) | ELISA, Neut | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0733-CN-S(P) | Human Anti-PGE2 Recombinant Antibody; scFv Fragment (HPAB-0733-CN-S(P)) | ELISA, Neut | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0731-CN-F(E) | Human Anti-PGE2 Recombinant Antibody; Fab Fragment (HPAB-0731-CN-F(E)) | ELISA, Neut | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0732-CN-F(E) | Human Anti-PGE2 Recombinant Antibody; Fab Fragment (HPAB-0732-CN-F(E)) | ELISA, Neut | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0733-CN-F(E) | Human Anti-PGE2 Recombinant Antibody; Fab Fragment (HPAB-0733-CN-F(E)) | ELISA, Neut | Humanized Fab |
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