Recombinant Human Anti-RSV Antibody Fab Fragment (D25) (CAT#: PFBL-322)

Figure 1 Recombinant Human Anti-RSV Antibody Fab Fragment (PFBL-322) in SDS-PAGE

Lane 1: Non-reduced Fab
Lane 2: Reduced Fab

Figure 2 Recombinant Human Anti-RSV Antibody Fab Fragment (PFBL-322) in HPLC

The purity of PFBL-322 was greater than 95% as determined by SEC-HPLC

Figure 3 Recombinant Human Anti-RSV Antibody Fab Fragment (PFBL-322) in WB

Western blot analysis of PFBL-322 was performed by loading Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PFBL-322 and HRP Goat Anti-Human IgG (H+L) as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (1 μg)

Figure 4 Recombinant Human Anti-RSV Antibody Fab Fragment (PFBL-322) in DB

Dot Blot analysis of PFBL-322 was performed by coating with Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag).

PFBL-322 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG (H+L) as a secondary antibody (1: 2000)

Figure 5 Recombinant Human Anti-RSV Antibody Fab Fragment (PFBL-322) in ELISA

ELISA analysis of PFBL-322 was performed by coating with Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag). Then blocked with BSA, and incubated with PFBL-322. The HRP-conjugated goat anti-human IgG (H+L) as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.