Recombinant Human Anti-RSV Antibody Fab Fragment (D25) (CAT#: PFBL-322)
Recombinant Human Antibody (D25) Fab Fragment is specific to RSV, expressed in Chinese Hamster Ovary cells (CHO).
Specific Inquiry
SDS-PAGE
Figure 1 Recombinant Human Anti-RSV Antibody Fab Fragment (PFBL-322) in SDS-PAGE
Lane 1: Non-reduced Fab
Lane 2: Reduced Fab
SEC-HPLC
Figure 2 Recombinant Human Anti-RSV Antibody Fab Fragment (PFBL-322) in HPLC
The purity of PFBL-322 was greater than 95% as determined by SEC-HPLC
WB
Figure 3 Recombinant Human Anti-RSV Antibody Fab Fragment (PFBL-322) in WB
Western blot analysis of PFBL-322 was performed by loading Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PFBL-322 and HRP Goat Anti-Human IgG (H+L) as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (1 μg)
DB
Figure 4 Recombinant Human Anti-RSV Antibody Fab Fragment (PFBL-322) in DB
Dot Blot analysis of PFBL-322 was performed by coating with Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag).
PFBL-322 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG (H+L) as a secondary antibody (1: 2000)
ELISA
Figure 5 Recombinant Human Anti-RSV Antibody Fab Fragment (PFBL-322) in ELISA
ELISA analysis of PFBL-322 was performed by coating with Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Protein (His Tag). Then blocked with BSA, and incubated with PFBL-322. The HRP-conjugated goat anti-human IgG (H+L) as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- Fab
- Specificity
- Tested positive against native RSV
- Species Reactivity
- RSV
- Clone
- D25
- Applications
- Can be useful in applications such as: Western blot; Enzyme-linked Immunosorbent Assay; Neutralization; Functional Study
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- RSV; Human respiratory syncytial virus
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone D25"
See other products for "RSV"
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-098 | Anti-RSV Recombinant Antibody (Felvizumab) | IF, IP, Neut, FuncS, ELISA, FC, ICC | IgG1 - kappa |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-538CL | Human Anti-RSV Recombinant Antibody (TAB-538CL) | ELISA | Human IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-693 | Human Anti-RSV Recombinant Antibody (clone B21m) | WB, IF, FuncS | Human IgG |
| HPAB-0363LY | Recombinant Humanized Anti-RSV Antibody (1308F) | Neutralization, ELISA | |
| HPAB-2527LY | Human Anti-RSV Recombinant Antibody (clone mab2) | ELISA, Neut | Human IgG |
| HPAB-2528LY | Human Anti-RSV Recombinant Antibody (clone mab27) | ELISA, Neut | Human IgG |
| HPAB-2529LY | Human Anti-RSV Recombinant Antibody (clone mab30) | ELISA, Neut | Human IgG |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-824LC | Anti-RSV Recombinant Antibody (HNK11) | ELISA | Mouse antibody |
| MRO-825LC | Anti-RSV Recombinant Antibody (HNK19) | ELISA | Mouse antibody |
| MRO-826LC | Anti-RSV Recombinant Antibody (HNK20) | ELISA | Mouse antibody |
| MRO-827LC | Anti-RSV Recombinant Antibody (HNK22) | ELISA | Mouse antibody |
| MRO-828LC | Anti-RSV Recombinant Antibody (HNK24) | ELISA | Mouse antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4397 | Hi-Affi™ Recombinant Rabbit Anti-RSV Monoclonal Antibody (SI488DS) | Neut | IgG |
| MOR-4398 | Hi-Affi™ Recombinant Rabbit Anti-RSV Monoclonal Antibody (SI489DS) | ELISA, FC, ICC/IF, IF | IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0114-CN-S(P) | Recombinant Humanized Anti-RSV Antibody scFv Fragment (HuRSV19) | ELISA, BL | Humanized scFv |
| HPAB-N0235-YC-S(P) | Human Anti-RSV Recombinant Antibody; scFv Fragment (HPAB-N0235-YC-S(P)) | Neut | Human scFv |
| HPAB-1908-FY-F(E) | Human Anti-RSV Recombinant Antibody (clone RSV22); scFv Fragment | Neut | Human scFv |
| HPAB-1591WJ-S(P) | Mouse Anti-RSV Recombinant Antibody; scFv Fragment (clone RS-348) | ELISA, WB, IF, Neut | Mouse scFv |
| HPAB-1592WJ-S(P) | Mouse Anti-RSV Recombinant Antibody; scFv Fragment (clone RS-18B2) | ELISA, WB, IF, Neut | Mouse scFv |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-098 | Afuco™ Anti-RSV ADCC Recombinant Antibody (Felvizumab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2527LY-F(E) | Human Anti-RSV Recombinant Antibody (clone mab2); Fab Fragment | ELISA | Human Fab |
| HPAB-2528LY-F(E) | Human Anti-RSV Recombinant Antibody (clone mab27); Fab Fragment | ELISA | Human Fab |
| HPAB-2529LY-F(E) | Human Anti-RSV Recombinant Antibody (clone mab30); Fab Fragment | ELISA | Human Fab |
| HPAB-2530LY-F(E) | Human Anti-RSV Recombinant Antibody; Fab Fragment (HPAB-2530LY-F(E)) | ELISA | Human Fab |
| HPAB-2531LY-F(E) | Human Anti-RSV Recombinant Antibody (clone mab41); Fab Fragment | ELISA | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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