Human Anti-RSV Recombinant Antibody (clone B21m) (CAT#: PABL-693)

Figure 1 Surface plasmon resonance (SPR) analysis using the ProteOn XPR36 in co-injection mode IgG1 variants.

(A) wild-type (WT); (B) N434A or (C) N434Y were diluted 1:5 in a concentration series from 1000 to 1.6 nM, injected over rat FcRn for 1 min in PBS-TE (pH 6.0) and followed immediately by a co-injection of PBS-TE (pH 7.4) without mAb for 3 min.

Cooper, P. R. , Kliwinski, C. M. , Perkinson, R. A. , Ragwan, E. , Mabus, J. R. , & Powers, G. D. , et al. (2014). The contribution of cell surface fcrn in monoclonal antibody serum uptake from the intestine in suckling rat pups. Frontiers in Pharmacology, 5.

Figure 2 Greatest serum levels of IgG measured when administered at site with greatest rat FcRn mRNA expression.

(A) Serum levels of the WT mAb were measured after 90 min following administration at the proximal small intestine (S.I; duodenal region) and at the distal S.I (ileum region) in suckling rat pups (n = 6). A one-way ANOVA followed by Bonferroni's multiple comparison test was carried out versus respective WT. (B) mRNA expression of rat FcRn, assessed using reverse transcription polymerase chain reaction (RT-PCR), from mucosal scrapings of proximal and distal small intestine, and proximal and distal colon. Data shown is fold change over proximal colon which had the lowest level of FcRn mRNA).

Cooper, P. R. , Kliwinski, C. M. , Perkinson, R. A. , Ragwan, E. , Mabus, J. R. , & Powers, G. D. , et al. (2014). The contribution of cell surface fcrn in monoclonal antibody serum uptake from the intestine in suckling rat pups. Frontiers in Pharmacology, 5.