Anti-Human SEMA4A Recombinant Antibody (161-70-1 ) (CAT#: TAB-426CT)
Recombinant monoclonal antibody 161-70-1 specifically binds to SEMA4A. It can inhibit T cell proliferation and Th2 differentiation induced by IL-4, anti-CD3, anti-CD28 and recombinant sema4A.
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SDS-PAGE
Figure 1 Anti-Human SEMA4A Recombinant Antibody (TAB-426CT) in SDS-PAGE
SDS-PAGE analysis of TAB-426CT in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. The non-reduced antibody migrates as about 150-250 kDa.
WB
Figure 2 Anti-Human SEMA4A Recombinant Antibody (TAB-426CT) in WB
Western blot analysis of TAB-426CT was performed by loading SEMA4A protein (Lane 1 0.5 μg, Lane 2 1 μg, Lane 3 1.5 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-426CT and HRP goat anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
ELISA
Figure 3 Anti-Human SEMA4A Recombinant Antibody (TAB-426CT) in ELISA
ELISA analysis of TAB-426CT was performed by coating with human SEMA4A protein, His tag. Then blocked with BSA and incubated with TAB-426CT. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Mouse
- Specificity
- Human
- Species Reactivity
- Human
- Clone
- 161-70-1
- Applications
- Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Flow Cytometry; Immunohistochemistry; Blocking
- Related Disease
- Human autoimmune disease, allergic disease, inflammatory disease, graft versus host disease and graft rejection.
Target
- Alternative Names
- SEMA4A; sema domain; immunoglobulin domain (Ig); transmembrane domain (TM) and short cytoplasmic domain; (semaphorin) 4A; RP35; SEMB; SEMAB; CORD10; semaphorin-4A; sema B; semaphorin-B; anti-SEMA4A
- Gene ID
- 64218
- UniProt ID
- Q9H3S1
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Humanized Antibody Products
Mouse Antibody Products
Neutralizing Antibody Products
Antibody Magnetic Beads
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-422CT | Anti-Human SEMA4A Recombinant Antibody (HuLB51) | WB, ELISA, FC | Humanized antibody |
| TAB-422CT-S(P) | Anti-Human SEMA4A Recombinant Antibody scFv Fragment (HuLB51) | WB, ELISA, FC | Humanized antibody |
| TAB-423CT-S(P) | Anti-Human SEMA4A Recombinant Antibody scFv Fragment (HuLB70) | WB, ELISA, FC | Humanized antibody |
| TAB-422CT-F(E) | Anti-Human SEMA4A Recombinant Antibody Fab Fragment (HuLB51) | WB, ELISA, FC | Humanized antibody |
| TAB-423CT-F(E) | Anti-Human SEMA4A Recombinant Antibody Fab Fragment (HuLB70) | WB, ELISA, FC | Humanized antibody |
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