Anti-Human ST14 Recombinant Antibody (M32)
CAT#: TAB-0731CL
Recombinant monoclonal antibody M32 is a mouse monoclonal antibody that specifically binds to human matriptase.
Published Data
Gene Expression
WB
Figure 1 Biotinylated protein was precipitated with monomeric avidin agarose, and the avidin-down protein was analyzed using SDS-PAGE and Western blotting.
The antibody used was M32 against total Matriptase, and the location of the molecular marker (kDa) is shown on the left. Proteins and complexes are indicated by arrows and sizes.
Friis, S., Godiksen, S., Bornholdt, J., Selzer-Plon, J., Rasmussen, H. B., Bugge, T. H., ... & Vogel, L. K. (2011). Transport via the transcytotic pathway makes prostasin available as a substrate for matriptase. Journal of Biological Chemistry, 286(7), 5793-5802.
WB
Figure 2 Matriptase is active on the basolateral plasma membrane but not intracellularly.
Samples were analyzed by SDSPAGE and Western blotting with antibodies against total malic enzyme (M32) and malic enzyme-HAI-1 complex (M69).
Friis, S., Godiksen, S., Bornholdt, J., Selzer-Plon, J., Rasmussen, H. B., Bugge, T. H., ... & Vogel, L. K. (2011). Transport via the transcytotic pathway makes prostasin available as a substrate for matriptase. Journal of Biological Chemistry, 286(7), 5793-5802.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47014/1549_C6_1_selected.jpg
Normal Tissue
Figure 2 Testis
Cells in seminiferous ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56258/131283_A_6_6.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (CACO-2, CAPAN-2, HaCaT, OE19, RPMI-8226, SK-BR-3, T-47d, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149418-ST14/subcellular
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Specifications
- Immunogen
- Matriptase/HAI-1 complex
- Host Species
- Mouse
- Specificity
- Human
- Clone
- M32
- Applications
- ELISA, WB
- Related Disease
- Breast cancer
Applications
- Application Notes
- The antibody was validated for ELISA, Western Blot. For details, refer to Published Data.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
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| MOB-923-S(P) | Recombinant Anti-human ST14 Antibody scFv Fragment | FC, Neut, Biosensors, FuncS | scFv |
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| PSBL-655 | Human Anti-ST14 Recombinant Antibody (clone A11); scFv Fragment | WB, IF, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0732CL | Human Anti-ST14 Recombinant Antibody (TAB-0732CL) | ELISA, IHC, FC | Human IgG |
| TAB-0732CL-S(P) | Human Anti-ST14 Recombinant Antibody; scFv Fragment (TAB-0732CL-S(P)) | ELISA, IHC, FC | Human scFv |
| TAB-0732CL-F(E) | Human Anti-ST14 Recombinant Antibody; Fab Fragment (TAB-0732CL-F(E)) | ELISA, IHC, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0731CL-S(P) | Anti-Human ST14 Recombinant Antibody scFv Fragment (M32) | ELISA | |
| TAB-0731CL-F(E) | Anti-Human ST14 Recombinant Antibody Fab Fragment (M32) | ELISA |
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| VS-0425-YC304 | Recombinant Anti-ST14 Vesicular Antibody, EV Displayed (VS-0425-YC304) | ELISA, FC, Cell-uptake |
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