Anti-Human ST14 Recombinant Antibody (M32)
CAT#: TAB-0731CL
Recombinant monoclonal antibody M32 is a mouse monoclonal antibody that specifically binds to human matriptase.
Published Data
Gene Expression
WB
Figure 1 Biotinylated protein was precipitated with monomeric avidin agarose, and the avidin-down protein was analyzed using SDS-PAGE and Western blotting.
The antibody used was M32 against total Matriptase, and the location of the molecular marker (kDa) is shown on the left. Proteins and complexes are indicated by arrows and sizes.
Friis, S., Godiksen, S., Bornholdt, J., Selzer-Plon, J., Rasmussen, H. B., Bugge, T. H., ... & Vogel, L. K. (2011). Transport via the transcytotic pathway makes prostasin available as a substrate for matriptase. Journal of Biological Chemistry, 286(7), 5793-5802.
WB
Figure 2 Matriptase is active on the basolateral plasma membrane but not intracellularly.
Samples were analyzed by SDSPAGE and Western blotting with antibodies against total malic enzyme (M32) and malic enzyme-HAI-1 complex (M69).
Friis, S., Godiksen, S., Bornholdt, J., Selzer-Plon, J., Rasmussen, H. B., Bugge, T. H., ... & Vogel, L. K. (2011). Transport via the transcytotic pathway makes prostasin available as a substrate for matriptase. Journal of Biological Chemistry, 286(7), 5793-5802.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47014/1549_C6_1_selected.jpg
Normal Tissue
Figure 2 Testis
Cells in seminiferous ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56258/131283_A_6_6.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (CACO-2, CAPAN-2, HaCaT, OE19, RPMI-8226, SK-BR-3, T-47d, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149418-ST14/subcellular
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Specifications
- Immunogen
- Matriptase/HAI-1 complex
- Host Species
- Mouse
- Antibody Isotype
- IgG
- Specificity
- Human
- Clone
- M32
- Applications
- ELISA, WB
- Related Disease
- Breast cancer
Applications
- Application Notes
- The antibody was validated for ELISA, Western Blot. For details, refer to Published Data.
Target
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Customer Review
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Accurate Results
The Anti-Human ST14 Recombinant Antibody (M32) has been a valuable asset in my ELISA and Western Blot experiments targeting human matriptase. Its recombinant nature and detailed product description allowed me to optimize my experimental protocols. The performance consistency and high purity results have been very encouraging.
Informative Product
I appreciated the detailed specifications provided for the ST14 antibody. The product clearly outlines its use in detecting human matriptase and offers guidance on titration and storage. My experiments benefited from its reproducible results in ELISA and Western Blot tests, making it an asset for research involving ST14 detection.
Q&As
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How well does the ST14 antibody perform in ELISA and Western Blot assays?
A: We have observed that the Anti-Human ST14 Recombinant Antibody (M32) consistently produces accurate results in ELISA and Western Blot applications. Its specific binding to human matriptase is well-documented, and the recombinant production process ensures high purity and reproducibility, as confirmed by published validation data.
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What experimental tips do you provide for using this antibody?
A: We suggest that researchers carefully titrate the antibody and follow the provided assay protocols to achieve optimal performance in detecting matriptase. The comprehensive product data, including specifics on the recombinant format and recommended storage conditions, facilitates reproducible results, making it a reliable choice for in vitro experiments on ST14.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-0731CL, RRID: AB_3714276)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| MOB-923-F(E) | Recombinant Anti-human ST14 Antibody Fab Fragment | ELISA, IP, FuncS | Fab |
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| PSBL-655 | Human Anti-ST14 Recombinant Antibody (clone A11); scFv Fragment | WB, IF, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
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| TAB-0732CL | Human Anti-ST14 Recombinant Antibody (TAB-0732CL) | ELISA, IHC, FC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0731CL-S(P) | Anti-Human ST14 Recombinant Antibody scFv Fragment (M32) | ELISA |
| CAT | Product Name | Application | Type |
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| TAB-0732CL-S(P) | Human Anti-ST14 Recombinant Antibody; scFv Fragment (TAB-0732CL-S(P)) | ELISA, IHC, FC | Human scFv |
| CAT | Product Name | Application | Type |
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| TAB-0731CL-F(E) | Anti-Human ST14 Recombinant Antibody Fab Fragment (M32) | ELISA |
| CAT | Product Name | Application | Type |
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| TAB-0732CL-F(E) | Human Anti-ST14 Recombinant Antibody; Fab Fragment (TAB-0732CL-F(E)) | ELISA, IHC, FC | Human Fab |
| CAT | Product Name | Application | Type |
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| VS-0224-XY123 | Mouse Anti-ST14 Recombinant Antibody (VS-0224-XY123) | ELISA, WB, IF, IP, IHC-P | Mouse IgG1 |
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| VS-0425-YC304 | Recombinant Anti-ST14 Vesicular Antibody, EV Displayed (VS-0425-YC304) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6933 | Anti-ST14 Immunohistochemistry Kit | IHC |
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