Human Anti-TAGLN Recombinant Antibody (clone Fab1630) (CAT#: HPAB-0127-LSX)
Recombinant monoclonal antibody to Transgelin. Clone Fab1630 is a human monoclonal antibody that can be potentially used in the treatment of atherogenic diseases.
Specific Inquiry
WB
Figure 1 Cross reactivity of representative Fabs from all patients with TAGLN and OMPs.
WB of purified human TAGLN (400 ng) with all representative Fabs (10 µg/mL). Unrelated human e8Fab-FLAGwas used as negative control. Anti-MYC-tag (C-terminal tag) and commercial anti-TAGLN were used as positive controls. While the commercial anti-TAGLN recognize selectively only one form of TAGLN, cloned human Fabs recognized both forms.
Canducci, F., Saita, D., Foglieni, C., Piscopiello, M. R., Chiesa, R., Colombo, A.,... & Burioni, R. (2012). Cross-reacting antibacterial auto-antibodies are produced within coronary atherosclerotic plaques of acute coronary syndrome patients. PloS one, 7(8).
WB
Figure 2 Cross reactivity of representative Fabs from all patients with TAGLN and OMPs.
WB of OmpK36 (500 ng) with all representative Fabs (10 ng/mL).
Canducci, F., Saita, D., Foglieni, C., Piscopiello, M. R., Chiesa, R., Colombo, A.,... & Burioni, R. (2012). Cross-reacting antibacterial auto-antibodies are produced within coronary atherosclerotic plaques of acute coronary syndrome patients. PloS one, 7(8).
ELISA
Figure 3 Cross reactivity of representative Fabs from all patients with TAGLN and OMPs.
ELISA with all representative Fabs on bacterial OMPs. Reactivity against bovine serum albumin (BSA) used as blocking antigen, is also shown.
Canducci, F., Saita, D., Foglieni, C., Piscopiello, M. R., Chiesa, R., Colombo, A.,... & Burioni, R. (2012). Cross-reacting antibacterial auto-antibodies are produced within coronary atherosclerotic plaques of acute coronary syndrome patients. PloS one, 7(8).
Specifications
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG
- Specificity
- Human TAGLN
- Species Reactivity
- Human
- Clone
- Fab1630
- Applications
- ELISA, WB
- Related Disease
- Atherogenic diseases
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot
The binding of representative Fabs248, Fab172, Fab1630 and Fab7816 to TAGLN was performed using 400 ng of purified TAGLN (Origene, TP302448) Myc-tagged loaded in each lane. TAGLN was run in SDS-PAGE and then transferred on PVDF membrane for 2 h at 0.350 A. PVDF was then blocked with PBS/10% BSA or PBS/10% Milk O/N at 4°C in agitation. Anti-Myc-Peroxidase and anti-human TAGLN commercial antibodies were used as positive controls. Purified Fabs were used in the assay. Anti-Light Chain K-Peroxidase antibody (PSB/5% BSA) and anti-Mouse-Fab antibody (PBS/Milk 5%) were used to reveal Fab binding or commercial anti-TAGLN binding respectively. After extensive washing with PBS/Tween-20 0.1% WB was developed with SuperSignal West Pico Chemiluminescent Substrate (Pierce).
Enzyme-Linked Immunosorbent Assay
8 µg of each OMP were coated on a 96 well ELISA plate in PBS, pH = 5.0 overnight at 4°C. The following day, the plate was blocked with PBS/BSA1%, and purified Fabs or freeze-and-thaw preparations were added (about 8 µg/ml) to each well. After washing with PBS/0.1% Tween-20, the anti-Human-Fab antibody-HRP (SIGMA-Aldrich), was added and incubated for 45–60 min at 37°C. After washing with PBS/0.1% Tween-20, 40 µL of TMB solution (Pierce) was added and the plate read at 450 nm.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1882CT | Recombinant Mouse anti-Human TAGLN Monoclonal antibody (8C8) | ELISA, WB | |
| FAMAB-1264CQ | Mouse Anti-TAGLN Recombinant Antibody (clone SAIC-33B-129) | WB, Immuno-MRM, ELISA | Mouse IgG1 |
| VS-0923-FY141 | Recombinant Mouse Anti-TAGLN Antibody (VS-0923-FY141) | ELISA, WB | Mouse IgG1 |
| VS3-XY3517 | Rabbit Anti-TAGLN Recombinant Antibody (clone R02-8A4) | WB, IHC-P | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0127-LSX-S(P) | Human Anti-TAGLN Recombinant Antibody (clone Fab1630); scFv Fragment | WB | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0127-LSX-F(E) | Human Anti-TAGLN Recombinant Antibody (clone Fab1630); Fab Fragment | WB | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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