Mouse Anti-TAGLN Recombinant Antibody (clone SAIC-33B-129)
CAT#: FAMAB-1264CQ
This product is a recombinant mouse antibody that recognizes TAGLN. The antibody was purified by affinity chromatography. It can be used in WB, Immuno-MRM, ELISA.
Published Data
Gene Expression
ELISA
Figure 1 ELISA data from screening recombinant monoclonal antibodies using recombinant proteins or overexpressed lysates.
ELISA data were reduced using Prism software to calculate the 50% binding point (B50%). The B50% values should not be interpreted as absolute numbers that define mAb performance (i.e., not analogously to affinity constants); instead, the B50% values can be used as relative values to compare the performances of the mAb within this group of mAbs.
Schoenherr, R. M., Saul, R. G., Whiteaker, J. R., Yan, P., Whiteley, G. R., & Paulovich, A. G. (2015). Anti-Peptide Monoclonal Antibodies Generated for Immuno-Multiple Reaction Monitoring-Mass Spectrometry Assays Have a High Probability of Supporting Western blot and ELISA*[S]. Molecular & cellular proteomics, 14(2), 382-398.
WB
Figure 2 Images of Western blots from screening the recombinant and hybridoma monoclonal antibodies (mAbs) using recombinant proteins, overexpressed lysates, and 6 breast cancer NCI60 cell line lysates.
The purified mAbs were run as controls alongside the recombinant proteins or the overexpressed lysate-proteins. Because the mAbs' protein targets (except for TSHB and CGB) were expected to be detected in breast cancer cell lines. For the Western blots using the NCI60 breast cancer cell lines.
Schoenherr, R. M., Saul, R. G., Whiteaker, J. R., Yan, P., Whiteley, G. R., & Paulovich, A. G. (2015). Anti-Peptide Monoclonal Antibodies Generated for Immuno-Multiple Reaction Monitoring-Mass Spectrometry Assays Have a High Probability of Supporting Western blot and ELISA*[S]. Molecular & cellular proteomics, 14(2), 382-398.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria & microtubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19467/235_G3_2_selected.jpg
Normal Tissue
Figure 1 Colon
Enterocytes - Peripheral nerve/ganglion Staining: Low Intensity: Weak
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19467/45371_A_9_3.jpg
Normal Tissue
Figure 2 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19467/45371_A_6_6.jpg
Normal Tissue
Figure 3 High expression in smooth muscle
Smooth muscle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19467/45371_B_4_7.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+ SV40 Large T+, CACO-2, EFO-21, fHDF/TERT166, hTERT-RPE1, LHCN-M2, U-138 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149591-TAGLN
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Specifications
- Immunogen
- Peptide AAEDYGVIK conjugated to KLH.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human TAGLN
- Species Reactivity
- Human
- Clone
- SAIC-33B-129
- Applications
- WB, Immuno-MRM, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone SAIC-33B-129"
- CAT
- Product Name
See other products for "TAGLN"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1882CT | Recombinant Mouse anti-Human TAGLN Monoclonal antibody (8C8) | ELISA, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0127-LSX | Human Anti-TAGLN Recombinant Antibody (clone Fab1630) | ELISA, WB | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0127-LSX-S(P) | Human Anti-TAGLN Recombinant Antibody (clone Fab1630); scFv Fragment | WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0127-LSX-F(E) | Human Anti-TAGLN Recombinant Antibody (clone Fab1630); Fab Fragment | WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0923-FY141 | Recombinant Mouse Anti-TAGLN Antibody (VS-0923-FY141) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-XY3517 | Rabbit Anti-TAGLN Recombinant Antibody (clone R02-8A4) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1175 | AbPlus™ Anti-TAGLN Magnetic Beads (VS-0724-YC1175) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1139 | Rabbit Anti-TAGLN Recombinant Antibody (VS13-YC1139) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2245 | Anti-TAGLN Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7082 | Anti-Mouse TAGLN Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7081 | Anti-Human TAGLN Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7083 | Anti-Rat TAGLN Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7084 | Anti-Porcine TAGLN Immunohistochemistry Kit | IHC |
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