Recombinant Mouse Anti-TGEV  S protein Antibody (1AF10) (CAT#: PABZ-212)

Figure 1 APN-binding domain and epitopes for neutralizing mAbs overlap in TGEV and PRCV S proteins.

C. Binding of site A-specific TGEV-neutralizing mAb to the SA protein. mAb binding to plastic-bound SA protein, monitored by optical density (OD). Site A mAbs are specific for the Aa (1BB1), Ab (1DE7) and Ac (6AC3, 1AF10) subsites [24]. An anti-HA mAb that binds to the HA tag in the SA protein was used as control. D. Site A-specific mAbs prevent SA protein binding to pAPN. Binding of the SA protein to BHK-pAPN cells in panel B was monitored alone or in the presence of site A (shown in C) or site D-specific (1DG3) mAb, and the binding ratio determined. C, D. Mean and standard deviation for three experiments.

Reguera, J., Santiago, C., Mudgal, G., Ordoño, D., Enjuanes, L., & Casasnovas, J. M. (2012). Structural bases of coronavirus attachment to host aminopeptidase N and its inhibition by neutralizing antibodies. PLoS pathogens, 8(8), e1002859.