Recombinant Mouse Anti-TGEV S protein Antibody (1AF10) (CAT#: PABZ-212)
Recombinant Mouse Antibody (1AF10) is capable of binding to TGEV S protein, expressed in Chinese Hamster Ovary cells (CHO). Antibody binding to RBD mutants showed that site A epitopes extend across the TGEV RBD tip, although there are some differences among the three A subsites. The epitopes recognized by Aa- and Ab-specific mAbs bear the exposed TGEV Trp571 residue at the β3–β4 loop, whereas epitopes for the Ac-specific mAbs center on Tyr528 in the β1–β2 turn.
Specific Inquiry
Figure 1 APN-binding domain and epitopes for neutralizing mAbs overlap in TGEV and PRCV S proteins.
C. Binding of site A-specific TGEV-neutralizing mAb to the SA protein. mAb binding to plastic-bound SA protein, monitored by optical density (OD). Site A mAbs are specific for the Aa (1BB1), Ab (1DE7) and Ac (6AC3, 1AF10) subsites [24]. An anti-HA mAb that binds to the HA tag in the SA protein was used as control. D. Site A-specific mAbs prevent SA protein binding to pAPN. Binding of the SA protein to BHK-pAPN cells in panel B was monitored alone or in the presence of site A (shown in C) or site D-specific (1DG3) mAb, and the binding ratio determined. C, D. Mean and standard deviation for three experiments.
Reguera, J., Santiago, C., Mudgal, G., Ordoño, D., Enjuanes, L., & Casasnovas, J. M. (2012). Structural bases of coronavirus attachment to host aminopeptidase N and its inhibition by neutralizing antibodies. PLoS pathogens, 8(8), e1002859.
Specifications
- Immunogen
- TGEV Spike protein
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native TGEV S protein
- Species Reactivity
- TGEV
- Clone
- 1AF10
- Applications
- Neut Assay-Dependent
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody 1AF10 has been reported in application of Neut. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
- Alternative Names
- Spike protein; S protein
- UniProt ID
- Q0PKZ5
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABZ-139 | Recombinant Human Anti-SARS S Antibody (80R) | Neut | IgG |
| PABL-719 | Recombinant Mouse Anti-TGEV Spike protein Antibody (1AF10) | ELISA, Neut | IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-139 | Recombinant Human Anti-SARS S Antibody Fab Fragment (80R) | Neut | Fab |
| PFBZ-212 | Recombinant Mouse Anti-TGEV S protein Antibody Fab Fragment (1AF10) | Neut | Fab |
| PSBL-712 | Recombinant Mouse Anti-TGEV Spike protein Antibody scFv Fragment (mAb 1AF10) | ELISA, Neut | scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-712 | Recombinant Mouse Anti-TGEV Spike protein Antibody Fab Fragment (mAb 1AF10) | ELISA, Neut | Fab |
| PSBZ-139 | Recombinant Human Anti-SARS S Antibody scFv Fragment (80R) | Neut | scFv |
| PSBZ-212 | Recombinant Mouse Anti-TGEV S protein Antibody scFv Fragment (1AF10) | Neut | scFv |
MHC Tetramer for Virology
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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