Recombinant Mouse Anti-TGEV Spike protein Antibody (1AF10)
CAT#: PABL-719
Recombinant Mouse Antibody (1AF10) is capable of binding to TGEV Spike protein , expressed in Chinese Hamster Ovary cells (CHO).
Published Data
Figure 1 APN-binding domain and epitopes for neutralizing mAbs overlap in TGEV and PRCV S proteins.
A . Scheme of TGEV and PRCV S proteins showing the S1, S2, transmembrane (T) and cytoplasmic (Cy) regions. Location of the C, B, D and A antigenic sites [21], and the pAPN RBD (bar with N and C-terminal residues) [22] are shown. Length is indicated for mature S1 regions. B. A short, soluble S protein variant containing the TGEV RBD region binds to cell surface pAPN. Binding of a bivalent SA-Fc fusion protein (SA) to BHK-pAPN (open histograms), alone (left) or in the presence of the site A-specific 6AC3 mAb (right), as analyzed in FACS. Filled histograms correspond to an unrelated Fc fusion protein. C. Binding of site A-specific TGEV- neutralizing mAb to the SA protein. mAb binding to plastic-bound SA protein, monitored by optical density (OD). Site A mAbs are specific for the Aa (1BB1), Ab (1DE7) and Ac (6AC3, 1AF10) subsites [24]. An anti-HA mAb that binds to the HA tag in the SA protein was used as control. D. Site A-specific mAbs prevent SA protein binding to pAPN. Binding of the SA protein to BHK-pAPN cells in panel B was monitored alone or in the presence of site A (shown in C) or site D-specific (1DG3) mAb, and the binding ratio determined (see Materials and Methods). C, D . Mean and standard deviation for three experiments.
Reguera, J., Santiago, C., Mudgal, G., Ordoño, D., Enjuanes, L., & Casasnovas, J. M. (2012). Structural bases of coronavirus attachment to host aminopeptidase N and its inhibition by neutralizing antibodies. PLoS pathogens, 8(8), e1002859.
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Specifications
- Immunogen
- TGEV Spike protein
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native TGEV Spike protein
- Species Reactivity
- TGEV
- Clone
- 1AF10
- Applications
- ELISA, Neut
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The S protein antibody has been reported in applications of Neutralization. The structure of the TGEV RBD-1AF10 Fab fragment was initially determined by the molecular replacement (MR) method using the PHASER program, and two search models having either the variable or constant regions of the PDB ID 1AIF mAb structure.
Target
- Alternative Names
- S protein; Spike protein
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| PABZ-212 | Recombinant Mouse Anti-TGEV S protein Antibody (1AF10) | Neut | IgG |
| CAT | Product Name | Application | Type |
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| PFBZ-139 | Recombinant Human Anti-SARS S Antibody Fab Fragment (80R) | Neut | Fab |
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| CAT | Product Name | Application | Type |
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| PFBL-712 | Recombinant Mouse Anti-TGEV Spike protein Antibody Fab Fragment (PFBL-712) | ELISA, Neut | Fab |
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