Human Anti-TNFSF15 Recombinant Antibody (TAB-345CQ) (CAT#: TAB-345CQ)
This product is a recombinant human antibody that recognizes TL1A (Tumor necrosis factor-like ligand 1A). The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
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SDS-PAGE
Figure 1 Human Anti-TNFSF15 Recombinant Antibody (TAB-345CQ) in SDS-PAGE
SDS-PAGE analysis of TAB-345CQ in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of TAB-345CQ was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 2 Human Anti-TNFSF15 Recombinant Antibody (TAB-345CQ) in HPLC
The purity of TAB-345CQ was 95.9931% as determined by SEC-HPLC.
WB
Figure 3 Human Anti-TNFSF15 Recombinant Antibody (TAB-345CQ) in WB
Western blot analysis of TAB-345CQ was performed by loading recombinant human TNFSF15 protein protein.
TAB-345CQ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 4 Human Anti-TNFSF15 Recombinant Antibody (TAB-345CQ) in ELISA
ELISA analysis of TAB-345CQ was performed by coating with recombinant human TNFSF15 protein. Then blocked with BSA, and incubated with Human Anti-TNFSF15 Recombinant Antibody (TAB-345CQ). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- TL1A protein
- Host Species
- Human
- Type
- Human IgG1, κ
- Specificity
- Human TL1A (Tumor necrosis factor-like ligand 1A)
- Species Reactivity
- Human, Cynomolgus monkey, Mouse
- Applications
- Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Flow Cytometry; Neutralization
- Related Disease
- Allergic airway disease
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- TNFSF15; Tumor necrosis factor superfamily member 15; TL1; TL1A; VEGI; VEGI192A; tumor necrosis factor ligand superfamily member 15; TNF superfamily ligand TL1A; TNF ligand-related molecule 1; vascular endothelial cell growth inhibitor; vascular endothelial growth inhibitor-192A
- Gene ID
- 9966
- UniProt ID
- O95150
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Mouse Antibodies
Humanized Antibodies
Human Antibodies
Neutralizing Antibodies
ScFv Antibodies
Fab Antibodies
IgG Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-334CQ | Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ) | ELISA | Mouse IgG2a, κ |
| TAB-335CQ | Mouse Anti-TNFSF15 Recombinant Antibody (TAB-335CQ) | ELISA, FC, BL | Mouse IgG2a, κ |
| TAB-338CQ | Hamster Anti-TNFSF15 Recombinant Antibody (TAB-338CQ) | ELISA, FC, Block | Hamster IgG |
| TAB-339CQ | Hamster Anti-TNFSF15 Recombinant Antibody (TAB-339CQ) | ELISA, FC, BL | Hamster IgG |
| TAB-346CQ | Mouse Anti-TNFSF15 Recombinant Antibody (TAB-346CQ) | ELISA | Mouse IgG |
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