Human Anti-TYRO3 Recombinant Antibody (clone 2709)
CAT#: HPAB-0028-DJB
The product recognizesthe extracelluar domain of huamn Tyro3 receptor. It can be used for treating paitentswith hyperproliferative or infectious diseases.
Published Data
Gene Expression
ELISA
Figure 1 ELISA Binding assay of scFvs/IgG against hTyro 3 ECD.
ELISA
Figure 2 The purified extracellular domains of hTyro 3, hAxl or hMer were coated on micro titerplate and incubated in the presence of anti-Tyro 3 scFvs (μg/mL).
Fig. A. 2410, 2411, 2412, 2413, 2414, 2415 scFvs. Fig. 4B. 2717 and 2718 scFvs. Fig. C. 2709, 2710, 2711 and 2713 scFvs. Bound fragments were revealed with an anti-Myc-HRP antibody.
ELISA
Figure 3 GAS6 competition assay with anti-Tyro3 scFvs.
Binding of scFvs and MAB859 to full-length Tyro3. Full-length Tyro3 was coated onto an ELISA microtiter plate. scFvs were used at 10 mg/ml and MAB859 was used at 3 concentrations: 1 mg/ml (MAB859-1), 5 mg/ml (MAB859-5) and 10 mg/ml (MAB859-10). scFv binding to full-length Tyro3 was detected with an anti-HIS-HRP antibody and MAB859 with an anti-mouse-HRP antibody. The negative controls with the detection antibodies alone were shown (anti-HIS or anti-mouse).
ELISA
Figure 4 Cross-reactivity against cynomolgus Tyro3 ECD of anti-Tyro3 in the scFv.
ELISA
Figure 5 Cross-reactivity against murine Tyro3 ECD of anti-Tyro3 in the scFv format.
ELISA
Figure 6 Epitope restriction of anti-Tyro3 scFvs to structural domains of Tyro3 ECD.
FC
Figure 7 Binding of anti-Tyro3 IgGs in living cells.
HEK293 cells transfected to express recombinant human Tyro3 and HEK293 untransfected cells were stained with the Live-Dead NIR dye and either incubated with the TYRO 3-specific MAB859 monoclonal antibody, its isotype control (upper panels) or to individual anti-Tyro3 IgG (middle and bottom panels). IgG isotype control is IGX2656. MAB002 and MAB859 binding was revealed with a PE-conjugated Goat anti-Mouse Ig Ab (BD Pharmingen). Anti-Tyro3 IgG binding was revealed with a PE-conjugated Mouse-anti Human Lambda Light Chain Ab. Data were acquired using an ARIA III flow cytometer (Becton Dickinson) and analyzed using the Kaluza software (Beckman Coulter). Histograms shown are restricted to live cells that show a characteristic size and granulometry of HEK293 cells. Anti-Tyro3 IgG Tyro3-specific binding is shown by fluorescence intensity increase of Tyro3-transfected cells (black line) compared to untransfected cells (grey line).
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Normal Tissue
Figure 1 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71245/153510_B_7_5.jpg
Normal Tissue
Figure 2 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71245/153510_A_9_3.jpg
Normal Tissue
Figure 3 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71245/153510_A_7_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71245/153510_A_6_6.jpg
Normal Tissue
Figure 5 Skin
Epidermal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71245/153510_B_4_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BEWO)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000092445-TYRO3
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Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human IgG
- Specificity
- Human TYRO3
- Species Reactivity
- Human
- Clone
- 2709
- Applications
- ELISA, FC
- Related Disease
- Hyperproliferative, infectious diseases
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 2709"
- CAT
- Product Name
See other products for "TYRO3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3735 | Rabbit Anti-TYRO3 Recombinant Antibody (clone DS3735AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-096CN | Human Anti-TYRO3 Recombinant Antibody (clone 2410) | ELISA, FC | Human IgG |
| NS-097CN | Human Anti-TYRO3 Recombinant Antibody (clone 2718) | ELISA, FC | Human IgG |
| NS-096CN-F(E) | Human Anti-TYRO3 Recombinant Antibody (clone 2410); Fab Fragment | ELISA, FC | Human Fab |
| NS-097CN-F(E) | Human Anti-TYRO3 Recombinant Antibody (clone 2718); Fab Fragment | ELISA, FC | Human Fab |
| NS-096CN-S(P) | Human Anti-TYRO3 Recombinant Antibody (clone 2410); scFv Fragment | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1526LY-S(P) | Human Anti-TYRO3 Recombinant Antibody (clone 2411); scfv Fragment | ELISA | Human scfv |
| HPAB-1527LY-S(P) | Human Anti-TYRO3 Recombinant Antibody (clone 2412); scfv Fragment | ELISA | Human scfv |
| HPAB-1528LY-S(P) | Human Anti-TYRO3 Recombinant Antibody (clone 2413); scfv Fragment | ELISA | Human scfv |
| HPAB-1529LY-S(P) | Human Anti-TYRO3 Recombinant Antibody (clone 2415); scfv Fragment | ELISA | Human scfv |
| HPAB-1530LY-S(P) | Human Anti-TYRO3 Recombinant Antibody (clone 2711); scfv Fragment | ELISA | Human scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1524LY-F(E) | Human Anti-TYRO3 Recombinant Antibody (clone 2710); Fab Fragment | ELISA | Human Fab |
| HPAB-1525LY-F(E) | Human Anti-TYRO3 Recombinant Antibody (clone 2717); Fab Fragment | ELISA | Human Fab |
| HPAB-1526LY-F(E) | Human Anti-TYRO3 Recombinant Antibody (clone 2411); Fab Fragment | ELISA | Human Fab |
| HPAB-1527LY-F(E) | Human Anti-TYRO3 Recombinant Antibody (clone 2412); Fab Fragment | ELISA | Human Fab |
| HPAB-1528LY-F(E) | Human Anti-TYRO3 Recombinant Antibody (clone 2413); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1209 | Mouse Anti-TYRO3 Recombinant Antibody (clone 1444CT895.86.31) | WB | Mouse IgG1, κ |
| VS3-QX1210 | Mouse Anti-TYRO3 Recombinant Antibody (clone 6D6F10) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY1503 | Recombinant Mouse Anti-TYRO3 Antibody (clone 2F5B8) | ELISA, IHC, FC | Mouse IgG1 |
| VS7-0425-WR1076 | Mouse Anti-TYRO3 Monoclonal Antibody (clone 1C10E8) | ICC | Mouse IgG1 |
| VS7-0425-WR1077 | Mouse Anti-TYRO3 Monoclonal Antibody (clone 7A10C11; 7A10G4) | WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2406 | Anti-TYRO3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7668 | Anti-Mouse TYRO3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7667 | Anti-Human TYRO3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC436 | Recombinant Anti-TYRO3 Vesicular Antibody, EV Displayed (VS-0425-YC436) | ELISA, FC, Cell-uptake |
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