Mouse Anti-Tau Recombinant Antibody; Fab Fragment (TAB-535CT-F(E)) (CAT#: TAB-535CT-F(E))

Figure 1 Anti-Human Tau Recombinant Antibody (TAB-535CT-F(E)) in SDS-PAGE

SDS-PAGE analysis of TAB-535CT-F(E) in non-reduced (Lane 1, 1.5 μg) and β-mercaptoethanol-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 25-35 kDa.

Figure 2 Anti-Human Tau Recombinant Antibody (TAB-535CT-F(E)) in SEC-HPLC

The purity of TAB-535CT-F(E) was 99.5904% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Anti-Human Tau Recombinant Antibody (TAB-535CT-F(E)) in WB

Western blot analysis of TAB-535CT-F(E) was performed with human Tau protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-535CT-F(E) and HRP conjugated Anti-Mouse IgG (H+L) as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 4 Anti-Human Tau Recombinant Antibody (TAB-535CT-F(E)) in DB

Dot Blot analysis of TAB-535CT-F(E) was performed by coating with human Tau protein (His tag).

TAB-535CT-F(E) incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG (H+L) as a secondary antibody (1: 2000)

Figure 5 Anti-Human Tau Recombinant Antibody (TAB-535CT-F(E)) in ELISA

ELISA analysis of TAB-535CT-F(E) was performed by coating with human Tau protein (His tag). Then blocked with BSA and incubated with TAB-535CT-F(E). The HRP conjugated Anti-Mouse IgG (H+L) as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.