Recombinant monoclonal antibody specifically binds to huma Tau. This antibody can be potentially used in treating Alzheimer's Disease.
Figure 1 Anti-Human Tau Recombinant Antibody (TAB-528CT) in SDS-PAGE
SDS-PAGE analysis of TAB-528CT in non-reduced (Lane 1, 1.5 μg) and β-mercaptoethanol-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 48-63 kDa and 20-25 kDa, respectively.
Figure 2 Anti-Human Tau Recombinant Antibody (TAB-528CT) in SEC-HPLC
The purity of TAB-528CT was 96.7229% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 3 Anti-Human Tau Recombinant Antibody (TAB-528CT) in WB
Western blot analysis of TAB-528CT was performed with human Tau protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-528CT and HRP conjugated Anti-Mouse IgG (H+L) as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 4 Anti-Human Tau Recombinant Antibody (TAB-528CT) in DB
Dot Blot analysis of TAB-528CT was performed by coating with human Tau protein (His tag).
TAB-528CT incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG (H+L) as a secondary antibody (1: 2000)
Figure 5 Anti-Human Tau Recombinant Antibody (TAB-528CT) in ELISA
ELISA analysis of TAB-528CT was performed by coating with human Tau protein (His tag). Then blocked with BSA and incubated with TAB-528CT. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-951-S(P) | Recombinant Human Anti-Tau Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
HPAB-2561LY-S(P) | Human Anti-Tau Recombinant Antibody; scFv Fragment (HPAB-2561LY-S(P)) | WB, IHC, ELISA | Human scFv |
HPAB-2562LY-S(P) | Human Anti-Tau Recombinant Antibody; scFv Fragment (HPAB-2562LY-S(P)) | WB, IHC, ELISA | Human scFv |
HPAB-2563LY-S(P) | Human Anti-Tau Recombinant Antibody; scFv Fragment (HPAB-2563LY-S(P)) | WB, IHC, ELISA | Humanized scfv |
HPAB-2564LY-S(P) | Human Anti-Tau Recombinant Antibody; scFv Fragment (HPAB-2564LY-S(P)) | WB | Humanized scfv |
There are currently no Customer reviews or questions for TAB-528CT. Click the button above to contact us or submit your feedback about this product.
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-528CT, RRID: AB_3111969)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.