Mouse Anti-UPAR Recombinant Antibody (clone ATN615)

Customer Review

There are currently no Customer reviews or questions for PABC-130. Click the button above to contact us or submit your feedback about this product.

Emily Walker
16.Jul,23

Excellent for uPAR Studies

The Anti-uPAR Recombinant Antibody has been excellent for our uPAR research. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.

John Martinez
12.Dec,22

Effective in Immunoassays

We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for uPAR is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.

Sarah Thompson
08.Sep,21

Consistent and Reliable

This antibody has proven to be consistent and reliable in our assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for uPAR have been particularly beneficial.

Q&As

1. Is the anti-UPAR recombinant antibody clone ATN615 suitable for use in Western blotting?

   A: Yes, the anti-UPAR recombinant antibody clone ATN615 (PABC-130) is suitable for use in Western blotting. It provides specific binding to UPAR, allowing for reliable detection in Western blot assays.

2. What are the storage recommendations for the anti-UPAR recombinant antibody clone ATN615?

   A: The recommended storage condition for the anti-UPAR recombinant antibody clone ATN615 (PABC-130) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

3. Can the anti-UPAR recombinant antibody clone ATN615 be used in immunoprecipitation assays?

   A: Yes, the anti-UPAR recombinant antibody clone ATN615 (PABC-130) can be used in immunoprecipitation assays. It provides specific binding to UPAR, enabling the successful precipitation of the target protein from complex mixtures.

4. Is the anti-UPAR recombinant antibody clone ATN615 effective in ELISA applications?

   A: Yes, the anti-UPAR recombinant antibody clone ATN615 (PABC-130) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of UPAR.

5. What is the optimal dilution for using the anti-UPAR recombinant antibody clone ATN615 in immunofluorescence?

   A: The optimal dilution for using the anti-UPAR recombinant antibody clone ATN615 (PABC-130) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Zhu, Ke, et al. "The D2D3 form of uPAR acts as an immunotoxin and may cause diabetes and kidney disease." Science Translational Medicine 15.714 (2023): eabq6492. https://doi.org/10.1126/scitranslmed.abq6492
   This study investigates the role of the D2D3 form of the soluble urokinase plasminogen activator receptor (suPAR) in the development of diabetes and kidney disease. The research found that D2D3 acts as an immunotoxin, leading to kidney disease and insulin-dependent diabetes mellitus in transgenic mice fed a high-fat diet. These mice exhibited symptoms such as microalbuminuria, elevated serum creatinine, glomerular hypertrophy, decreased insulin and C-peptide levels, impaired glucose-stimulated insulin secretion, and reduced pancreatic β cell mass. The study demonstrated that targeting D2D3 could potentially serve as a therapeutic approach for treating kidney diseases and insulin-dependent diabetes mellitus.
   Creative Biolabs played a crucial role in this study by providing the anti-uPAR monoclonal antibodies used for immunoprecipitation and Western blot analyses. Specifically, the anti-uPAR antibody ATN615 (Cat#: PABC-130) from Creative Biolabs was utilized to detect the presence of D2D3 in human sera and mouse samples. These products were instrumental in identifying the pathogenic role of D2D3 and validating the effectiveness of anti-uPAR antibody treatment in restoring β cell mass and function in D2D3 transgenic mice. The contributions of Creative Biolabs' reagents were vital in enabling the detailed examination of D2D3's impact on kidney and pancreatic functions.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABC-130, RRID: AB_3111577)

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "Clone ATN615"

See other products for "uPAR"

Select a product category from the dropdown menu below to view related products.