Rabbit Anti-VCP Recombinant Antibody (clone DS3832AB)
CAT#: MOR-3832
Recombinant Rabbit anti-VCP monoclonal antibody for WB, IHC, ICC, IP, FC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12728/96_C10_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12814/97_E6_2_selected.jpg
Normal Tissue
Figure 3 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12728/30453_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12728/30453_A_5_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12728/30453_A_9_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12728/30453_B_7_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12728/30453_A_7_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12728/30453_A_9_4.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165280-VCP
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Specifications
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human VCP
- Species Reactivity
- Human
- Clone
- DS3832AB
- Applications
- WB, IHC, ICC, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "VCP"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2175MZ | Recombinant Mouse Anti-Human VCP Antibody (clone 5H0) | ICC, IF, IHC-P, IP, WB | Mouse antibody |
| ZG-0619U | Rabbit Anti-VCP Recombinant Antibody (clone 5H12) | ELISA, WB, IHC, IF, IP | Rabbit IgG |
| VS3-QX1232 | Mouse Anti-VCP Recombinant Antibody (clone 1344CT150.163.114) | WB, ICC | Mouse IgG1, κ |
| VS3-FY1541 | Recombinant Rabbit Anti-VCP Antibody (clone R06-9D7) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS7-0425-WR1109 | Mouse Anti-VCP Recombinant Antibody (clone 8E4A4) | WB, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2464 | Anti-VCP Immunohistochemistry Kit | IHC | |
| VS-0525-XY7828 | Anti-Rat VCP Immunohistochemistry Kit | IHC | |
| VS-0525-XY7827 | Anti-Human VCP Immunohistochemistry Kit | IHC |
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