Rabbit Anti-VCP Recombinant Antibody (clone 5H12) (CAT#: ZG-0619U)

Figure 1 Rabbit Anti-VCP Antibody (ZG-0619U) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, Rat brain tissue, Rat liver tissue
All lanes: VCP antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa

Figure 2 Rabbit Anti-VCP Antibody (ZG-0619U) in IHC

IHC image of ZG-0619U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Figure 3 Rabbit Anti-VCP Antibody (ZG-0619U) in IHC

IHC image of ZG-0619U diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Figure 4 Rabbit Anti-VCP Antibody (ZG-0619U) in IF

Immunofluorescence staining of SY5Y Cells with ZG-0619U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Figure 5 Rabbit Anti-VCP Antibody (ZG-0619U) in IP

Immunoprecipitating VCP in U251 whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0619U in U251 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0619U(2µg)+ U251 whole cell lysate(500µg)
Lane 3: U251 whole cell lysate (10µg)