PDZ domain scaffold protein anti-Human PTEN (CAT#: SPD-L152)
The PDZ domain that bound PTEN was isolated from the library using phage display techonology. PDZ domains are modular protein-interaction domains that are specialized for binding to short peptide motifs at the extreme carboxy (C) termini of other proteins, athough they can also have other modes of interaction. After affinity maturation, PDZ domain variants were identified having affinities for PTEN as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31335/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31335/if_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31335/134179_A_5_6_selected.jpg
(Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31335/134179_A_5_6_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4076/12125_B_8_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4076/12125_B_8_5.jpg
Figure 4 Lymph node
(Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4076/12125_A_8_8.jpg
(Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4076/12125_A_8_8.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171862-PTEN/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171862-PTEN/subcellular
Specifications
- Scaffold Name
- PDZ domain
- Origin
- MAGI-2 (membrane associated guanylate kinase inverted-2)
- Target
- PTEN
- Species Reactivity
- Human
- Expression Host
- BL21 (DE3) cells
- Applications
- ELISA; IHC; Microscopy; FC; WB; FuncS
Target
- Alternative Names
- MAGI-2 (membrane associated guanylate kinase inverted-2); PDZ domain; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
- Gene ID
- 5728
- UniProt ID
- P60484
Related Resources
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Downloads
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IgG Antibody Products
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Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1640z | Mouse Anti-PTEN Recombinant Antibody (clone 27C11) | WB, ELISA, FC, ICC, IF | Mouse IgG1 |
| MOB-0338MZ | Recombinant Mouse Anti-Human PTEN Antibody (clone 7I3.2) | IHC | Mouse antibody |
| VS3-CJ1061 | Mouse Anti-PTEN Recombinant Antibody (VS3-CJ1061) | WB | Mouse IgG1, κ |
| VS3-CJ1062 | Rabbit Anti-PTEN Recombinant Antibody (VS3-CJ1062) | WB, ICC, IF, IHC | Rabbit IgG |
| VS3-XY1314 | Mouse Anti-PTEN Recombinant Antibody (clone 4D8) | WB, IP, IF | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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