Recombinant Mouse Anti-Heat Shock Protein-25 and Heat Shock Protein 27 Antibody (clone ISP25-19)
CAT#: MOB-0125MZ
This antibody is a recombinant mouse monoclonal antibody which specifically reacts with Heat Shock Protein-25 and Heat Shock Protein 27.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47330/if_selected.jpg
Normal Tissue
Figure 2 Esophagus
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4439/17421_B_4_1_val_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes -
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_A_7_5.jpg
Normal Tissue
Figure 5 High expression in vagina
Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_B_2_2.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (hTCEpi, MCF7)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106211-HSPB1
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Specifications
- Immunogen
- Recombinant chicken HSP-25
- Host Species
- Mouse
- Type
- Mouse antibody
- Antibody Isotype
- IgG2b
- Specificity
- Heat Shock Protein-25 and Heat Shock Protein 27
- Species Reactivity
- Chicken
- Clone
- ISP25-19
- Applications
- ELISA, WB, IF, IP
Target
- Alternative Names
- HSPB1; Heat Shock Protein-25 and Heat Shock Protein 27
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Great Specificity
The recombinant mouse antibody clone ISP25-19 targeting HSP25 and HSP27 is exceptional in specificity. It performs reliably in ELISA, WB, IF, and IP applications. I appreciate the detailed documentation on its immunoglobulin subclass and the purification method used, which supports high sensitivity and reproducibility in heat shock protein research.
Consistent Performance
Our lab has found the anti-HSP25/HSP27 antibody to consistently deliver accurate results in various assays. The product insights regarding its expression in CHO cells and the recommended storage practices have minimized variability. This consistency in performance makes it a valuable tool for studying stress response proteins.
Q&As
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How specific is the ISP25-19 clone for HSP25 and HSP27?
A: We have validated that the ISP25-19 clone reacts specifically with both HSP25 and HSP27. Its recombinant expression and purification from CHO systems yield a high purity product, thus ensuring that it produces consistent signals in ELISA, WB, IF and IP applications. The product specifications highlight its strong performance across various experimental setups.
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What applications are ideal for this antibody?
A: We have optimized the anti-HSP25/HSP27 antibody for multiple research applications, including ELISA, WB, IF, and IP. The product details ensure that users receive clear instructions for use and storage, resulting in a consistent performance in detecting heat shock proteins during stress response studies.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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